1d4c: Difference between revisions

Revision as of 13:26, 2 May 2008

CRYSTAL STRUCTURE OF THE UNCOMPLEXED FORM OF THE FLAVOCYTOCHROME C FUMARATE REDUCTASE OF SHEWANELLA PUTREFACIENS STRAIN MR-1

OverviewOverview

Fumarate respiration is one of the most widespread types of anaerobic respiration. The soluble fumarate reductase of Shewanella putrefaciens MR-1 is a periplasmic tetraheme flavocytochrome c. The crystal structures of the enzyme were solved to 2.9 A for the uncomplexed form and to 2.8 A and 2.5 A for the fumarate and the succinate-bound protein, respectively. The structures reveal a flexible capping domain linked to the FAD-binding domain. A catalytic mechanism for fumarate reduction based on the structure of the complexed protein is proposed. The mechanism for the reverse reaction is a model for the homologous succinate dehydrogenase (complex II) of the respiratory chain. In flavocytochrome c fumarate reductase, all redox centers are in van der Waals contact with one another, thus providing an efficient conduit of electrons from the hemes via the FAD to fumarate.

About this StructureAbout this Structure

1D4C is a Single protein structure of sequence from Shewanella putrefaciens. Full crystallographic information is available from OCA.

ReferenceReference

Structure and mechanism of the flavocytochrome c fumarate reductase of Shewanella putrefaciens MR-1., Leys D, Tsapin AS, Nealson KH, Meyer TE, Cusanovich MA, Van Beeumen JJ, Nat Struct Biol. 1999 Dec;6(12):1113-7. PMID:10581551 Page seeded by OCA on Fri May 2 13:26:10 2008

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OCA

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 [[Image:1d4c.jpg|left|200px]]
- {{Structure
+<!--
-|PDB= 1d4c |SIZE=350|CAPTION= <scene name='initialview01'>1d4c</scene>, resolution 2.9&Aring;
+The line below this paragraph, containing "STRUCTURE_1d4c", creates the "Structure Box" on the page.
-|SITE=
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Succinate_dehydrogenase Succinate dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.3.99.1 1.3.99.1] </span>
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-|GENE=
+-->
-|DOMAIN=
+{{STRUCTURE_1d4c|  PDB=1d4c  |  SCENE=  }}
-|RELATEDENTRY=[[1d4c|1D4C]], [[1d4d|1D4D]], [[1d4e|1D4E]]
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1d4c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1d4c OCA], [http://www.ebi.ac.uk/pdbsum/1d4c PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1d4c RCSB]</span>
-}}
 '''CRYSTAL STRUCTURE OF THE UNCOMPLEXED FORM OF THE FLAVOCYTOCHROME C FUMARATE REDUCTASE OF SHEWANELLA PUTREFACIENS STRAIN MR-1'''
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 [[Category: Meyer, T E.]]
 [[Category: Tsapin, A S.]]
-[[Category: tetraheme flavocytochrome c]]
+[[Category: Tetraheme flavocytochrome c]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 13:26:10 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 19:34:25 2008''