1lf6: Difference between revisions
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==CRYSTAL STRUCTURE OF BACTERIAL GLUCOAMYLASE== | ==CRYSTAL STRUCTURE OF BACTERIAL GLUCOAMYLASE== | ||
<StructureSection load='1lf6' size='340' side='right' caption='[[1lf6]], [[Resolution|resolution]] 2.10Å' scene=''> | <StructureSection load='1lf6' size='340' side='right' caption='[[1lf6]], [[Resolution|resolution]] 2.10Å' scene=''> | ||
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1ayx|1ayx]], [[3gly|3gly]], [[1lf9|1lf9]]</td></tr> | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1ayx|1ayx]], [[3gly|3gly]], [[1lf9|1lf9]]</td></tr> | ||
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glucan_1,4-alpha-glucosidase Glucan 1,4-alpha-glucosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.3 3.2.1.3] </span></td></tr> | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glucan_1,4-alpha-glucosidase Glucan 1,4-alpha-glucosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.3 3.2.1.3] </span></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1lf6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1lf6 OCA], [http://pdbe.org/1lf6 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1lf6 RCSB], [http://www.ebi.ac.uk/pdbsum/1lf6 PDBsum]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1lf6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1lf6 OCA], [http://pdbe.org/1lf6 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1lf6 RCSB], [http://www.ebi.ac.uk/pdbsum/1lf6 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1lf6 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Evolutionary Conservation == | == Evolutionary Conservation == | ||
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Check<jmol> | Check<jmol> | ||
<jmolCheckbox> | <jmolCheckbox> | ||
<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/lf/1lf6_consurf.spt"</scriptWhenChecked> | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/lf/1lf6_consurf.spt"</scriptWhenChecked> | ||
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | ||
<text>to colour the structure by Evolutionary Conservation</text> | <text>to colour the structure by Evolutionary Conservation</text> | ||
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</div> | </div> | ||
<div class="pdbe-citations 1lf6" style="background-color:#fffaf0;"></div> | <div class="pdbe-citations 1lf6" style="background-color:#fffaf0;"></div> | ||
== References == | == References == | ||
<references/> | <references/> | ||
Revision as of 00:40, 25 January 2018
CRYSTAL STRUCTURE OF BACTERIAL GLUCOAMYLASECRYSTAL STRUCTURE OF BACTERIAL GLUCOAMYLASE
Structural highlights
Evolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMedThe first crystal structures of a two-domain, prokaryotic glucoamylase were determined to high resolution from the clostridial species Thermoanaerobacterium thermosaccharolyticum with and without acarbose. The N-terminal domain has 18 antiparallel strands arranged in beta-sheets of a super-beta-sandwich. The C-terminal domain is an (alpha/alpha)(6) barrel, lacking the peripheral subdomain of eukaryotic glucoamylases. Interdomain contacts are common to all prokaryotic Family GH15 proteins. Domains similar to those of prokaryotic glucoamylases in maltose phosphorylases (Family GH65) and glycoaminoglycan lyases (Family PL8) suggest evolution from a common ancestor. Eukaryotic glucoamylases may have evolved from prokaryotic glucoamylases by the substitution of the N-terminal domain with the peripheral subdomain and by the addition of a starch-binding domain. Crystal structure and evolution of a prokaryotic glucoamylase.,Aleshin AE, Feng PH, Honzatko RB, Reilly PJ J Mol Biol. 2003 Mar 14;327(1):61-73. PMID:12614608[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References |
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