3b6r: Difference between revisions
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==Crystal structure of Human Brain-type Creatine Kinase== | ==Crystal structure of Human Brain-type Creatine Kinase== | ||
<StructureSection load='3b6r' size='340' side='right' caption='[[3b6r]], [[Resolution|resolution]] 2.00Å' scene=''> | <StructureSection load='3b6r' size='340' side='right' caption='[[3b6r]], [[Resolution|resolution]] 2.00Å' scene=''> | ||
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">CKB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr> | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">CKB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr> | ||
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Creatine_kinase Creatine kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.3.2 2.7.3.2] </span></td></tr> | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Creatine_kinase Creatine kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.3.2 2.7.3.2] </span></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3b6r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3b6r OCA], [http://pdbe.org/3b6r PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3b6r RCSB], [http://www.ebi.ac.uk/pdbsum/3b6r PDBsum]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3b6r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3b6r OCA], [http://pdbe.org/3b6r PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3b6r RCSB], [http://www.ebi.ac.uk/pdbsum/3b6r PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3b6r ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Function == | == Function == | ||
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</div> | </div> | ||
<div class="pdbe-citations 3b6r" style="background-color:#fffaf0;"></div> | <div class="pdbe-citations 3b6r" style="background-color:#fffaf0;"></div> | ||
== References == | == References == | ||
<references/> | <references/> | ||
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[[Category: Atp-binding]] | [[Category: Atp-binding]] | ||
[[Category: Brain type]] | [[Category: Brain type]] | ||
[[Category: Cytoplasm]] | |||
[[Category: Dimeric]] | [[Category: Dimeric]] | ||
[[Category: Kinase]] | [[Category: Kinase]] | ||
[[Category: Nucleotide-binding]] | [[Category: Nucleotide-binding]] | ||
[[Category: Phosphorylation]] | [[Category: Phosphorylation]] | ||
[[Category: Polymorphism]] | |||
[[Category: Transferase]] | [[Category: Transferase]] | ||
Revision as of 10:56, 25 October 2017
Crystal structure of Human Brain-type Creatine KinaseCrystal structure of Human Brain-type Creatine Kinase
Structural highlights
Function[KCRB_HUMAN] Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa. Evolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMedCreatine kinase is a member of the phosphagen kinase family, which catalyzes the reversible phosphoryl transfer reaction that occurs between ATP and creatine to produce ADP and phosphocreatine. Here, three structural aspects of human-brain-type-creatine-kinase (hBB-CK) were identified by X-ray crystallography: the ligand-free-form at 2.2A; the ADP-Mg2+, nitrate, and creatine complex (transition-state-analogue complex; TSAC); and the ADP-Mg2+-complex at 2.0A. The structures of ligand-bound hBB-CK revealed two different monomeric states in a single homodimer. One monomer is a closed form, either bound to TSAC or the ADP-Mg2+-complex, and the second monomer is an unliganded open form. These structural studies provide a detailed mechanism indicating that the binding of ADP-Mg2+ alone may trigger conformational changes in hBB-CK that were not observed with muscle-type-CK. Structural studies of human brain-type creatine kinase complexed with the ADP-Mg2+-NO3- -creatine transition-state analogue complex.,Bong SM, Moon JH, Nam KH, Lee KS, Chi YM, Hwang KY FEBS Lett. 2008 Nov 26;582(28):3959-65. Epub 2008 Oct 31. PMID:18977227[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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