2ow4: Difference between revisions
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==Crystal structure of a lectin from Canavalia maritima seeds (ConM) in complex with man1-2man-OMe== | ==Crystal structure of a lectin from Canavalia maritima seeds (ConM) in complex with man1-2man-OMe== | ||
<StructureSection load='2ow4' size='340' side='right' caption='[[2ow4]], [[Resolution|resolution]] 1.60Å' scene=''> | <StructureSection load='2ow4' size='340' side='right' caption='[[2ow4]], [[Resolution|resolution]] 1.60Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[2ow4]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/ | <table><tr><td colspan='2'>[[2ow4]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Beach_bean Beach bean]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2OW4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2OW4 FirstGlance]. <br> | ||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand= | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=MMA:O1-METHYL-MANNOSE'>MMA</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr> | ||
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2ovu|2ovu]], [[2p2k|2p2k]]</td></tr> | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2ovu|2ovu]], [[2p2k|2p2k]]</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ow4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ow4 OCA], [http://pdbe.org/2ow4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2ow4 RCSB], [http://www.ebi.ac.uk/pdbsum/2ow4 PDBsum]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ow4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ow4 OCA], [http://pdbe.org/2ow4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2ow4 RCSB], [http://www.ebi.ac.uk/pdbsum/2ow4 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2ow4 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Function == | == Function == | ||
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</div> | </div> | ||
<div class="pdbe-citations 2ow4" style="background-color:#fffaf0;"></div> | <div class="pdbe-citations 2ow4" style="background-color:#fffaf0;"></div> | ||
== References == | == References == | ||
<references/> | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: | [[Category: Beach bean]] | ||
[[Category: Azevedo, W F.de]] | [[Category: Azevedo, W F.de]] | ||
[[Category: Benevides, R G]] | [[Category: Benevides, R G]] | ||
Revision as of 12:11, 18 October 2017
Structural highlights
Function[CONA_CANRO] Glucose/D-mannose specific lectin. Evolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMedPlant lectins, especially those purified from species of the Leguminosae family, represent the best studied group of carbohydrate-binding proteins. The legume lectins from Diocleinae subtribe are highly similar proteins that present significant differences in the potency/efficacy of their biological activities. The structural studies of the interactions between lectins and sugars may clarify the origin of the distinct biological activities observed in this high similar class of proteins. In this way, this work presents a crystallographic study of the ConM and CGL (agglutinins from Canavalia maritima and Canavalia gladiata, respectively) in the following complexes: ConM/CGL:Man(alpha1-2)Man(alpha1-O)Me, ConM/CGL:Man(alpha1-3)Man(alpha1-O)Me and ConM/CGL:Man(alpha1-4)Man(alpha1-O)Me, which crystallized in different conditions and space group from the native proteins. The structures were solved by molecular replacement, presenting satisfactory values for R(factor) and R(free). Comparisons between ConM, CGL and ConA (Canavalia ensiformis lectin) binding mode with the dimannosides in subject, presented different interactions patterns, which may account for a structural explanation of the distincts biological properties observed in the lectins of Diocleinae subtribe. Structural analysis of Canavalia maritima and Canavalia gladiata lectins complexed with different dimannosides: new insights into the understanding of the structure-biological activity relationship in legume lectins.,Bezerra GA, Oliveira TM, Moreno FB, de Souza EP, da Rocha BA, Benevides RG, Delatorre P, de Azevedo WF Jr, Cavada BS J Struct Biol. 2007 Nov;160(2):168-76. Epub 2007 Aug 16. PMID:17881248[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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