1cx0: Difference between revisions

Revision as of 13:12, 2 May 2008

HEPATITIS DELTA VIRUS RIBOZYME

OverviewOverview

The self-cleaving ribozyme of the hepatitis delta virus (HDV) is the only catalytic RNA known to be required for the viability of a human pathogen. We obtained crystals of a 72-nucleotide, self-cleaved form of the genomic HDV ribozyme that diffract X-rays to 2.3 A resolution by engineering the RNA to bind a small, basic protein without affecting ribozyme activity. The co-crystal structure shows that the compact catalytic core comprises five helical segments connected as an intricate nested double pseudoknot. The 5'-hydroxyl leaving group resulting from the self-scission reaction is buried deep within an active-site cleft produced by juxtaposition of the helices and five strand-crossovers, and is surrounded by biochemically important backbone and base functional groups in a manner reminiscent of protein enzymes.

About this StructureAbout this Structure

1CX0 is a Single protein structure of sequence from Hepatitis delta virus. Full crystallographic information is available from OCA.

ReferenceReference

Crystal structure of a hepatitis delta virus ribozyme., Ferre-D'Amare AR, Zhou K, Doudna JA, Nature. 1998 Oct 8;395(6702):567-74. PMID:9783582 Page seeded by OCA on Fri May 2 13:12:18 2008

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OCA

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 [[Image:1cx0.gif|left|200px]]
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 '''HEPATITIS DELTA VIRUS RIBOZYME'''
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 [[Category: Doudna, J A.]]
 [[Category: Zhou, K.]]
-[[Category: nested double pseudoknot rna structure]]
+[[Category: Nested double pseudoknot rna structure]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 13:12:18 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 19:30:17 2008''