1cww: Difference between revisions

Revision as of 13:12, 2 May 2008

SOLUTION STRUCTURE OF THE CASPASE RECRUITMENT DOMAIN (CARD) FROM APAF-1

OverviewOverview

Activation of procaspase-9, a key component of the apoptosis mechanism, requires the interaction of its caspase recruitment domain (CARD) with the CARD in the adaptor protein Apaf-1. Using nuclear magnetic resonance spectroscopy and mutagenesis we have determined the structure of the CARD from Apaf-1 and the residues important for binding the CARD in procaspase-9. Apaf-1's CARD contains seven short alpha-helices with the core six helices arranged in an antiparallel manner. Residues in helix 2 have a central role in mediating interaction with procaspase-9 CARD. This interaction surface is distinct from that proposed based on the structure of the CARD from RAIDD, but is coincident with that of the structurally similar FADD death effector domain and the Apaf-1 CARD interface identified by crystallographic studies.

About this StructureAbout this Structure

1CWW is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

ReferenceReference

Solution structure and mutagenesis of the caspase recruitment domain (CARD) from Apaf-1., Day CL, Dupont C, Lackmann M, Vaux DL, Hinds MG, Cell Death Differ. 1999 Nov;6(11):1125-32. PMID:10578182 Page seeded by OCA on Fri May 2 13:12:01 2008

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OCA

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 [[Image:1cww.gif|left|200px]]
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 '''SOLUTION STRUCTURE OF THE CASPASE RECRUITMENT DOMAIN (CARD) FROM APAF-1'''
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 [[Category: Lackmann, M.]]
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-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 19:30:10 2008''