3epp: Difference between revisions

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==Crystal structure of mRNA cap guanine-N7 methyltransferase (RNMT) in complex with sinefungin==
==Crystal structure of mRNA cap guanine-N7 methyltransferase (RNMT) in complex with sinefungin==
<StructureSection load='3epp' size='340' side='right' caption='[[3epp]], [[Resolution|resolution]] 2.41&Aring;' scene=''>
<StructureSection load='3epp' size='340' side='right' caption='[[3epp]], [[Resolution|resolution]] 2.41&Aring;' scene=''>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">RNMT, KIAA0398 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">RNMT, KIAA0398 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/mRNA_(guanine-N(7)-)-methyltransferase mRNA (guanine-N(7)-)-methyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.1.56 2.1.1.56] </span></td></tr>
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/mRNA_(guanine-N(7)-)-methyltransferase mRNA (guanine-N(7)-)-methyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.1.56 2.1.1.56] </span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3epp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3epp OCA], [http://pdbe.org/3epp PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3epp RCSB], [http://www.ebi.ac.uk/pdbsum/3epp PDBsum]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3epp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3epp OCA], [http://pdbe.org/3epp PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3epp RCSB], [http://www.ebi.ac.uk/pdbsum/3epp PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3epp ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==

Revision as of 12:17, 25 October 2017

Crystal structure of mRNA cap guanine-N7 methyltransferase (RNMT) in complex with sinefunginCrystal structure of mRNA cap guanine-N7 methyltransferase (RNMT) in complex with sinefungin

Structural highlights

3epp is a 2 chain structure with sequence from Human. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:
Gene:RNMT, KIAA0398 (HUMAN)
Activity:mRNA (guanine-N(7)-)-methyltransferase, with EC number 2.1.1.56
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[MCES_HUMAN] mRNA-capping methyltransferase that methylates the N7 position of the added guanosine to the 5'-cap structure of mRNAs. Binds RNA containing 5'-terminal GpppC.[1] [2] [3] [4]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

References

  1. ↑ Tsukamoto T, Shibagaki Y, Niikura Y, Mizumoto K. Cloning and characterization of three human cDNAs encoding mRNA (guanine-7-)-methyltransferase, an mRNA cap methylase. Biochem Biophys Res Commun. 1998 Oct 9;251(1):27-34. PMID:9790902 doi:http://dx.doi.org/S0006-291X(98)99402-5
  2. ↑ Pillutla RC, Yue Z, Maldonado E, Shatkin AJ. Recombinant human mRNA cap methyltransferase binds capping enzyme/RNA polymerase IIo complexes. J Biol Chem. 1998 Aug 21;273(34):21443-6. PMID:9705270
  3. ↑ Saha N, Schwer B, Shuman S. Characterization of human, Schizosaccharomyces pombe, and Candida albicans mRNA cap methyltransferases and complete replacement of the yeast capping apparatus by mammalian enzymes. J Biol Chem. 1999 Jun 4;274(23):16553-62. PMID:10347220
  4. ↑ Gonatopoulos-Pournatzis T, Dunn S, Bounds R, Cowling VH. RAM/Fam103a1 is required for mRNA cap methylation. Mol Cell. 2011 Nov 18;44(4):585-96. doi: 10.1016/j.molcel.2011.08.041. PMID:22099306 doi:http://dx.doi.org/10.1016/j.molcel.2011.08.041

3epp, resolution 2.41Å

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