1ckv: Difference between revisions
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'''STRUCTURE OF THE SOLUBLE METHANE MONOOXYGENASE REGULATORY PROTEIN B''' | '''STRUCTURE OF THE SOLUBLE METHANE MONOOXYGENASE REGULATORY PROTEIN B''' | ||
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[[Category: Wagner, G.]] | [[Category: Wagner, G.]] | ||
[[Category: Walters, K J.]] | [[Category: Walters, K J.]] | ||
[[Category: | [[Category: Hydroxylase regulatory protein]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 12:50:38 2008'' | |||
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Revision as of 12:50, 2 May 2008
STRUCTURE OF THE SOLUBLE METHANE MONOOXYGENASE REGULATORY PROTEIN B
OverviewOverview
The soluble methane monooxygenase (sMMO; EC 1.14.13.25) from the pseudothermophile Methylococcus capsulatus (Bath) is a three-component enzyme system that catalyzes the selective oxidation of methane to methanol. We have used NMR spectroscopy to produce a highly refined structure of MMOB, the 16-kDa regulatory protein of this system. This structure has a unique and intricate fold containing seven beta-strands forming two beta-sheets oriented perpendicular to each other and bridged by three alpha-helices. The rate and efficiency of the methane hydroxylation by sMMO depend on dynamic binding interactions of the hydroxylase with the reductase and regulatory protein components during catalysis. We have monitored by NMR the binding of MMOB to the hydroxylase in the presence and absence of the reductase. The results of these studies provide structural insight into how the regulatory protein interacts with the hydroxylase.
About this StructureAbout this Structure
1CKV is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
ReferenceReference
Structure of the soluble methane monooxygenase regulatory protein B., Walters KJ, Gassner GT, Lippard SJ, Wagner G, Proc Natl Acad Sci U S A. 1999 Jul 6;96(14):7877-82. PMID:10393915 Page seeded by OCA on Fri May 2 12:50:38 2008