1nk4: Difference between revisions
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==GUANINE-GUANINE MISMATCH AT THE POLYMERASE ACTIVE SITE== | ==GUANINE-GUANINE MISMATCH AT THE POLYMERASE ACTIVE SITE== | ||
<StructureSection load='1nk4' size='340' side='right' caption='[[1nk4]], [[Resolution|resolution]] 1.60Å' scene=''> | <StructureSection load='1nk4' size='340' side='right' caption='[[1nk4]], [[Resolution|resolution]] 1.60Å' scene=''> | ||
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1njw|1njw]], [[1njx|1njx]], [[1njy|1njy]], [[1njz|1njz]], [[1nk0|1nk0]], [[1nk5|1nk5]], [[1nk6|1nk6]], [[1nk7|1nk7]], [[1nk8|1nk8]], [[1nk9|1nk9]], [[1nkb|1nkb]], [[1nkc|1nkc]], [[1nke|1nke]]</td></tr> | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1njw|1njw]], [[1njx|1njx]], [[1njy|1njy]], [[1njz|1njz]], [[1nk0|1nk0]], [[1nk5|1nk5]], [[1nk6|1nk6]], [[1nk7|1nk7]], [[1nk8|1nk8]], [[1nk9|1nk9]], [[1nkb|1nkb]], [[1nkc|1nkc]], [[1nke|1nke]]</td></tr> | ||
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/DNA-directed_DNA_polymerase DNA-directed DNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.7 2.7.7.7] </span></td></tr> | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/DNA-directed_DNA_polymerase DNA-directed DNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.7 2.7.7.7] </span></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1nk4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1nk4 OCA], [http://pdbe.org/1nk4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1nk4 RCSB], [http://www.ebi.ac.uk/pdbsum/1nk4 PDBsum]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1nk4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1nk4 OCA], [http://pdbe.org/1nk4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1nk4 RCSB], [http://www.ebi.ac.uk/pdbsum/1nk4 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1nk4 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Function == | == Function == | ||
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Check<jmol> | Check<jmol> | ||
<jmolCheckbox> | <jmolCheckbox> | ||
<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/nk/1nk4_consurf.spt"</scriptWhenChecked> | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/nk/1nk4_consurf.spt"</scriptWhenChecked> | ||
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | ||
<text>to colour the structure by Evolutionary Conservation</text> | <text>to colour the structure by Evolutionary Conservation</text> | ||
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</div> | </div> | ||
<div class="pdbe-citations 1nk4" style="background-color:#fffaf0;"></div> | <div class="pdbe-citations 1nk4" style="background-color:#fffaf0;"></div> | ||
== References == | == References == | ||
<references/> | <references/> | ||
Revision as of 10:29, 7 February 2018
GUANINE-GUANINE MISMATCH AT THE POLYMERASE ACTIVE SITEGUANINE-GUANINE MISMATCH AT THE POLYMERASE ACTIVE SITE
Structural highlights
Function[DPO1_GEOSE] In addition to polymerase activity, this DNA polymerase exhibits 5' to 3' exonuclease activity. Evolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMedAccurate DNA replication is essential for genomic stability. One mechanism by which high-fidelity DNA polymerases maintain replication accuracy involves stalling of the polymerase in response to covalent incorporation of mismatched base pairs, thereby favoring subsequent mismatch excision. Some polymerases retain a "short-term memory" of replication errors, responding to mismatches up to four base pairs in from the primer terminus. Here we a present a structural characterization of all 12 possible mismatches captured at the growing primer terminus in the active site of a polymerase. Our observations suggest four mechanisms that lead to mismatch-induced stalling of the polymerase. Furthermore, we have observed the effects of extending a mismatch up to six base pairs from the primer terminus and find that long-range distortions in the DNA transmit the presence of the mismatch back to the enzyme active site, suggesting the structural basis for the short-term memory of replication errors. Structures of mismatch replication errors observed in a DNA polymerase.,Johnson SJ, Beese LS Cell. 2004 Mar 19;116(6):803-16. PMID:15035983[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References |
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