1ceg: Difference between revisions

Revision as of 12:38, 2 May 2008

CEPHALOTHIN COMPLEXED WITH DD-PEPTIDASE

OverviewOverview

Two clinically-important beta-lactam antibiotics, cephalothin and cefotaxime, have been observed by X-ray crystallography bound to the reactive Ser62 of the D-alanyl-D-alanine carboxypeptidase/transpeptidase of Streptomyces sp. R61. Refinement of the two crystal structures produced R factors for 3 sigma (F) data of 0.166 (to 1.8 A) and 0.170 (to 2.0 A) for the cephalothin and cefotaxime complexes, respectively. In each complex, a water molecule is within 3.1 and 3.6 A of the acylated beta-lactam carbonyl carbon atom, but is poorly activated by active site residues for nucleophilic attack and deacylation. This apparent lack of good stereochemistry for facile hydrolysis is in accord with the long half-lives of cephalosporin intermediates in solution (20-40 h) and the efficacy of these beta-lactams as inhibitors of bacterial cell wall synthesis. Different hydrogen binding patterns of the two cephalosporins to Thr301 are consistent with the low cefotaxime affinity of an altered penicillin-binding protein, PBP-2x, reported in cefotaxime-resistant strains of Streptococcus pneumoniae, and with the ability of mutant class A beta-lactamases to hydrolyze third-generation cephalosporins.

About this StructureAbout this Structure

1CEG is a Single protein structure of sequence from Streptomyces sp.. Full crystallographic information is available from OCA.

ReferenceReference

Binding of cephalothin and cefotaxime to D-ala-D-ala-peptidase reveals a functional basis of a natural mutation in a low-affinity penicillin-binding protein and in extended-spectrum beta-lactamases., Kuzin AP, Liu H, Kelly JA, Knox JR, Biochemistry. 1995 Jul 25;34(29):9532-40. PMID:7626623 Page seeded by OCA on Fri May 2 12:38:27 2008

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OCA

@@ Line 1: / Line 1: @@
 [[Image:1ceg.gif|left|200px]]
- {{Structure
+<!--
-|PDB= 1ceg |SIZE=350|CAPTION= <scene name='initialview01'>1ceg</scene>, resolution 1.80&Aring;
+The line below this paragraph, containing "STRUCTURE_1ceg", creates the "Structure Box" on the page.
-|SITE= <scene name='pdbsite=ACT:Those+For+S.+R61+Are+Listed+Below'>ACT</scene>
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=CEP:CEPHALOTHIN+GROUP'>CEP</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Serine-type_D-Ala-D-Ala_carboxypeptidase Serine-type D-Ala-D-Ala carboxypeptidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.16.4 3.4.16.4] </span>
+or leave the SCENE parameter empty for the default display.
-|GENE=
+-->
-|DOMAIN=
+{{STRUCTURE_1ceg|  PDB=1ceg  |  SCENE=  }}
-|RELATEDENTRY=
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ceg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ceg OCA], [http://www.ebi.ac.uk/pdbsum/1ceg PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1ceg RCSB]</span>
-}}
 '''CEPHALOTHIN COMPLEXED WITH DD-PEPTIDASE'''
@@ Line 28: / Line 25: @@
 [[Category: Knox, J R.]]
 [[Category: Kuzin, A P.]]
-[[Category: d-amino acid peptidase]]
+[[Category: D-amino acid peptidase]]
-[[Category: hydrolase-transpeptidase]]
+[[Category: Hydrolase-transpeptidase]]
-[[Category: penicillin target]]
+[[Category: Penicillin target]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 12:38:27 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 19:20:06 2008''