1bs1: Difference between revisions

Revision as of 11:53, 2 May 2008

DETHIOBIOTIN SYNTHETASE COMPLEXED WITH DETHIOBIOTIN, ADP , INORGANIC PHOSPHATE AND MAGNESIUM

OverviewOverview

The crystal structures of two complexes of dethiobiotin synthetase, enzyme-diaminopelargonic acid-MgADP-AlF3 and enzyme-dethiobiotin-MgADP-Pi, respectively, have been determined to 1.8 A resolution. In dethiobiotin synthetase, AlF3 together with carbamylated diaminopelargonic acid mimics the phosphorylated reaction intermediate rather than the transition state complex for phosphoryl transfer. Observed differences in the binding of substrate, diaminopelargonic acid, and the product, dethiobiotin, suggest considerable displacements of substrate atoms during the ring closure step of the catalytic reaction. In both complexes, two metal ions are observed at the active site, providing evidence for a two-metal mechanism for this enzyme.

About this StructureAbout this Structure

1BS1 is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

ReferenceReference

Crystal structure of two quaternary complexes of dethiobiotin synthetase, enzyme-MgADP-AlF3-diaminopelargonic acid and enzyme-MgADP-dethiobiotin-phosphate; implications for catalysis., Kack H, Sandmark J, Gibson KJ, Schneider G, Lindqvist Y, Protein Sci. 1998 Dec;7(12):2560-6. PMID:9865950 Page seeded by OCA on Fri May 2 11:53:09 2008

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OCA

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 [[Image:1bs1.jpg|left|200px]]
- {{Structure
+<!--
-|PDB= 1bs1 |SIZE=350|CAPTION= <scene name='initialview01'>1bs1</scene>, resolution 1.8&Aring;
+The line below this paragraph, containing "STRUCTURE_1bs1", creates the "Structure Box" on the page.
-|SITE=
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=ADP:ADENOSINE-5&#39;-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=DAA:8-AMINO-7-CARBOXYAMINO-NONANOIC+ACID+WITH+ALUMINUM+FLUORIDE'>DAA</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Dethiobiotin_synthase Dethiobiotin synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.3.3.3 6.3.3.3] </span>
+or leave the SCENE parameter empty for the default display.
-|GENE=
+-->
-|DOMAIN=
+{{STRUCTURE_1bs1|  PDB=1bs1  |  SCENE=  }}
-|RELATEDENTRY=
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1bs1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1bs1 OCA], [http://www.ebi.ac.uk/pdbsum/1bs1 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1bs1 RCSB]</span>
-}}
 '''DETHIOBIOTIN SYNTHETASE COMPLEXED WITH DETHIOBIOTIN, ADP , INORGANIC PHOSPHATE AND MAGNESIUM'''
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 [[Category: Sandmark, J.]]
 [[Category: Schneider, G.]]
-[[Category: aluminum flouride]]
+[[Category: Aluminum flouride]]
-[[Category: atp-binding]]
+[[Category: Atp-binding]]
-[[Category: biotin biosynthesis]]
+[[Category: Biotin biosynthesis]]
-[[Category: ligase]]
+[[Category: Ligase]]
-[[Category: phosphoryl transfer]]
+[[Category: Phosphoryl transfer]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 11:53:09 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 19:07:12 2008''