1brl: Difference between revisions

Revision as of 11:52, 2 May 2008

THREE-DIMENSIONAL STRUCTURE OF BACTERIAL LUCIFERASE FROM VIBRIO HARVEYI AT 2.4 ANGSTROMS RESOLUTION

OverviewOverview

Luciferases are a class of enzymes that generate light in the visible spectrum. Luciferase from luminous marine bacteria is an alpha-beta heterodimer monooxygenase that catalyzes the oxidation of FMNH2 and a long-chain aliphatic aldehyde. The X-ray crystal structure of bacterial luciferase from Vibrio harveyi has been determined to 2.4 A resolution. The structure was solved by a combination of multiple isomorphous replacement and molecular averaging between the two heterodimers in the asymmetric unit. Each subunit folds into a (beta/alpha)8 barrel motif, and dimerization is mediated through a parallel four-helix bundle centered on a pseudo 2-fold axis that relates the structurally similar subunits. The vicinity of the active site has been identified on the alpha subunit by correlations with similar protein motifs and previous biochemical studies. The structure presented here represents the first molecular model of a bioluminescent enzyme.

About this StructureAbout this Structure

1BRL is a Protein complex structure of sequences from Vibrio harveyi. The following page contains interesting information on the relation of 1BRL with [Luciferase]. Full crystallographic information is available from OCA.

ReferenceReference

Three-dimensional structure of bacterial luciferase from Vibrio harveyi at 2.4 A resolution., Fisher AJ, Raushel FM, Baldwin TO, Rayment I, Biochemistry. 1995 May 23;34(20):6581-6. PMID:7756289 Page seeded by OCA on Fri May 2 11:52:17 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

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 [[Image:1brl.jpg|left|200px]]
- {{Structure
+<!--
-|PDB= 1brl |SIZE=350|CAPTION= <scene name='initialview01'>1brl</scene>, resolution 2.4&Aring;
+The line below this paragraph, containing "STRUCTURE_1brl", creates the "Structure Box" on the page.
-|SITE=
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Alkanal_monooxygenase_(FMN-linked) Alkanal monooxygenase (FMN-linked)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.14.14.3 1.14.14.3] </span>
+or leave the SCENE parameter empty for the default display.
-|GENE=
+-->
-|DOMAIN=
+{{STRUCTURE_1brl|  PDB=1brl  |  SCENE=  }}
-|RELATEDENTRY=
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1brl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1brl OCA], [http://www.ebi.ac.uk/pdbsum/1brl PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1brl RCSB]</span>
-}}
 '''THREE-DIMENSIONAL STRUCTURE OF BACTERIAL LUCIFERASE FROM VIBRIO HARVEYI AT 2.4 ANGSTROMS RESOLUTION'''
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 ==Reference==
 Three-dimensional structure of bacterial luciferase from Vibrio harveyi at 2.4 A resolution., Fisher AJ, Raushel FM, Baldwin TO, Rayment I, Biochemistry. 1995 May 23;34(20):6581-6. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/7756289 7756289]
-[[Category: Alkanal monooxygenase (FMN-linked)]]
 [[Category: Luciferase]]
 [[Category: Protein complex]]
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 [[Category: Fisher, A J.]]
 [[Category: Rayment, I.]]
-[[Category: monooxygenase]]
+[[Category: Monooxygenase]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 11:52:17 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 19:06:46 2008''