1bp7: Difference between revisions

Revision as of 11:47, 2 May 2008

GROUP I MOBILE INTRON ENDONUCLEASE I-CREI COMPLEXED WITH HOMING SITE DNA

OverviewOverview

The structure of the LAGLIDADG intron-encoded homing endonuclease I-CreI bound to homing site DNA has been determined. The interface is formed by an extended, concave beta sheet from each enzyme monomer that contacts each DNA half-site, resulting in direct side-chain contacts to 18 of the 24 base pairs across the full-length homing site. The structure indicates that I-CreI is optimized to its role in genetic transposition by exhibiting long site-recognition while being able to cleave many closely related target sequences. DNA cleavage is mediated by a compact pair of active sites in the I-CreI homodimer, each of which contains a separate bound divalent cation.

About this StructureAbout this Structure

1BP7 is a Single protein structure of sequence from Chlamydomonas reinhardtii. Full crystallographic information is available from OCA.

ReferenceReference

DNA recognition and cleavage by the LAGLIDADG homing endonuclease I-CreI., Jurica MS, Monnat RJ Jr, Stoddard BL, Mol Cell. 1998 Oct;2(4):469-76. PMID:9809068 Page seeded by OCA on Fri May 2 11:47:34 2008

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OCA

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 [[Image:1bp7.gif|left|200px]]
- {{Structure
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+The line below this paragraph, containing "STRUCTURE_1bp7", creates the "Structure Box" on the page.
-|SITE=
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=DA:2&#39;-DEOXYADENOSINE-5&#39;-MONOPHOSPHATE'>DA</scene>, <scene name='pdbligand=DC:2&#39;-DEOXYCYTIDINE-5&#39;-MONOPHOSPHATE'>DC</scene>, <scene name='pdbligand=DG:2&#39;-DEOXYGUANOSINE-5&#39;-MONOPHOSPHATE'>DG</scene>, <scene name='pdbligand=DT:THYMIDINE-5&#39;-MONOPHOSPHATE'>DT</scene>
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-|GENE=
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-|DOMAIN=
+{{STRUCTURE_1bp7|  PDB=1bp7  |  SCENE=  }}
-|RELATEDENTRY=
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1bp7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1bp7 OCA], [http://www.ebi.ac.uk/pdbsum/1bp7 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1bp7 RCSB]</span>
-}}
 '''GROUP I MOBILE INTRON ENDONUCLEASE I-CREI COMPLEXED WITH HOMING SITE DNA'''
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 [[Category: Jurica, M S.]]
 [[Category: Stoddard, B L.]]
-[[Category: chloroplast dna]]
+[[Category: Chloroplast dna]]
-[[Category: dna complex]]
+[[Category: Dna complex]]
-[[Category: endonuclease]]
+[[Category: Endonuclease]]
-[[Category: group i mobile intron]]
+[[Category: Group i mobile intron]]
-[[Category: intron homing]]
+[[Category: Intron homing]]
-[[Category: laglidadg motif]]
+[[Category: Laglidadg motif]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 11:47:34 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 19:05:28 2008''