1bb6: Difference between revisions

Revision as of 11:17, 2 May 2008

LYSOZYME COMPLEX WITH 4-METHYL-UMBELLIFERYL CHITOTRIOSE

OverviewOverview

Two complexes between rainbow trout lysozyme (RBTL) and 4-methylumbelliferyl chitobioside, 4MeU-(GlcNAc)2, and chitotrioside, 4MeU-(GlcNAc)3, were produced by co-crystallization and soaking, respectively, and the crystal structures were solved at 2.0 A resolution. The results show that 4-MeU-(GlcNAc)3 binds in subsites A-D and that 4-MeU-(GlcNAc)2 binds in subsites B-D in the active-site cleft of RBTL. This agrees well with earlier crystallographic studies on the binding of oligosaccharides of chitin to RBTL, which showed that (GlcNAc)3 binds to sites B-D in RBTL and not to A-C as seen in the human and turkey egg-white lysozymes. For both complexes the 4-MeU moiety in site D has diffuse electron density and is flexible, as it is only bound to water molecules and not to the protein. Since no electron density was observed in site E, the solved structures give views of nonproductive enzyme-substrate complexes.

About this StructureAbout this Structure

1BB6 is a Single protein structure of sequence from Oncorhynchus mykiss. Full crystallographic information is available from OCA.

ReferenceReference

Structural studies on the binding of 4-methylumbelliferone glycosides of chitin to rainbow trout lysozyme., Vollan VB, Hough E, Karlsen S, Acta Crystallogr D Biol Crystallogr. 1999 Jan;55(Pt 1):60-6. Epub 1999 Jan, 1. PMID:10089395 Page seeded by OCA on Fri May 2 11:17:54 2008

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OCA

@@ Line 1: / Line 1: @@
 [[Image:1bb6.gif|left|200px]]
- {{Structure
+<!--
-|PDB= 1bb6 |SIZE=350|CAPTION= <scene name='initialview01'>1bb6</scene>, resolution 2.0&Aring;
+The line below this paragraph, containing "STRUCTURE_1bb6", creates the "Structure Box" on the page.
-|SITE= <scene name='pdbsite=NUL:Catalytically+Active+Residues'>NUL</scene>
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=UMG:METHYL-UMBELLIFERTL-N-ACETYL-CHITOTRIOSE'>UMG</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17] </span>
+or leave the SCENE parameter empty for the default display.
-|GENE=
+-->
-|DOMAIN=
+{{STRUCTURE_1bb6|  PDB=1bb6  |  SCENE=  }}
-|RELATEDENTRY=
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1bb6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1bb6 OCA], [http://www.ebi.ac.uk/pdbsum/1bb6 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1bb6 RCSB]</span>
-}}
 '''LYSOZYME COMPLEX WITH 4-METHYL-UMBELLIFERYL CHITOTRIOSE'''
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 [[Category: Karlsen, S.]]
 [[Category: Vollan, V B.]]
-[[Category: hydrolase]]
+[[Category: Hydrolase]]
-[[Category: n-acetyl-muramidase]]
+[[Category: N-acetyl-muramidase]]
-[[Category: umbelliferone glycoside]]
+[[Category: Umbelliferone glycoside]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 11:17:54 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 18:57:25 2008''