1au7: Difference between revisions

Revision as of 10:41, 2 May 2008

PIT-1 MUTANT/DNA COMPLEX

OverviewOverview

Pit-1, a member of the POU domain family of transcription factors, characterized by a bipartite DNA-binding domain, serves critical developmental functions based on binding to diverse DNA elements in its target genes. Here we report a high resolution X-ray analysis of the Pit-1 POU domain bound to a DNA element as a homodimer. This analysis reveals that Pit-1 subdomains bind to perpendicular faces of the DNA, rather than opposite faces of the DNA as in Oct-1. This is accomplished by different spacing and orientation of the POU-specific domain. Contrary to previous predictions, the dimerization interface involves the carboxyl terminus of the DNA recognition helix of the homeodomain, which in an extended conformation interacts with specific residues at the amino terminus of helix alpha1 and in the loop between helices alpha3 and alpha4 of the POU-specific domain of the symmetry related monomer. These features suggest the molecular basis of disease-causing mutations in Pit-1 and provide potential basis for the flexible allostery between protein domains and DNA sites in the activation of target genes.

About this StructureAbout this Structure

1AU7 is a Single protein structure of sequence from Rattus norvegicus. Full crystallographic information is available from OCA.

ReferenceReference

Structure of Pit-1 POU domain bound to DNA as a dimer: unexpected arrangement and flexibility., Jacobson EM, Li P, Leon-del-Rio A, Rosenfeld MG, Aggarwal AK, Genes Dev. 1997 Jan 15;11(2):198-212. PMID:9009203 Page seeded by OCA on Fri May 2 10:41:46 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

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 [[Image:1au7.gif|left|200px]]
- {{Structure
+<!--
-|PDB= 1au7 |SIZE=350|CAPTION= <scene name='initialview01'>1au7</scene>, resolution 2.300&Aring;
+The line below this paragraph, containing "STRUCTURE_1au7", creates the "Structure Box" on the page.
-|SITE=
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=DA:2&#39;-DEOXYADENOSINE-5&#39;-MONOPHOSPHATE'>DA</scene>, <scene name='pdbligand=DC:2&#39;-DEOXYCYTIDINE-5&#39;-MONOPHOSPHATE'>DC</scene>, <scene name='pdbligand=DG:2&#39;-DEOXYGUANOSINE-5&#39;-MONOPHOSPHATE'>DG</scene>, <scene name='pdbligand=DT:THYMIDINE-5&#39;-MONOPHOSPHATE'>DT</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY=
+or leave the SCENE parameter empty for the default display.
-|GENE= PIT-1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10116 Rattus norvegicus])
+-->
-|DOMAIN=
+{{STRUCTURE_1au7|  PDB=1au7  |  SCENE=  }}
-|RELATEDENTRY=
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1au7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1au7 OCA], [http://www.ebi.ac.uk/pdbsum/1au7 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1au7 RCSB]</span>
-}}
 '''PIT-1 MUTANT/DNA COMPLEX'''
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 [[Category: Li, P.]]
 [[Category: Rosenfeld, M G.]]
-[[Category: complex (dna-binding protein/dna)]]
+[[Category: Cphd]]
-[[Category: cphd]]
+[[Category: Pituitary]]
-[[Category: pituitary]]
+[[Category: Pou domain]]
-[[Category: pou domain]]
+[[Category: Transcription factor]]
-[[Category: transcription factor]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 10:41:46 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 18:47:32 2008''