1at6: Difference between revisions

Revision as of 10:40, 2 May 2008

HEN EGG WHITE LYSOZYME WITH A ISOASPARTATE RESIDUE

OverviewOverview

The isomerization of Asp101 to isoaspartate autocatalytically proceeds via a succinimide intermediate in hen egg-white lysozyme at a mildly acidic condition. The crystal structures of succinimide and isoaspartate forms of the lysozyme proteins, each complexed with a tri-N-acetylchitotriose ligand, have been determined at 1.8 A resolution, and distinctively elucidate coplanar cyclic aminosuccinyl and beta-linked isoaspartyl residues. Compared with the liganded native protein with normal Asp101, succinimide 101 protrudes toward the ligand, and isoaspartate 101 extends away from the ligand. The formations of these residues caused the loss of three hydrogen-bonds between the ligand and the side-chains of Asp101 and Asn103 along with 0.5 A displacement of the ligand location.

About this StructureAbout this Structure

1AT6 is a Single protein structure of sequence from Gallus gallus. Full crystallographic information is available from OCA.

ReferenceReference

Succinimide and isoaspartate residues in the crystal structures of hen egg-white lysozyme complexed with tri-N-acetylchitotriose., Noguchi S, Miyawaki K, Satow Y, J Mol Biol. 1998 Apr 24;278(1):231-8. PMID:9571046 Page seeded by OCA on Fri May 2 10:40:00 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

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 [[Image:1at6.gif|left|200px]]
- {{Structure
+<!--
-|PDB= 1at6 |SIZE=350|CAPTION= <scene name='initialview01'>1at6</scene>, resolution 1.80&Aring;
+The line below this paragraph, containing "STRUCTURE_1at6", creates the "Structure Box" on the page.
-|SITE= <scene name='pdbsite=IAS:ASP+101+Isomerized+To+Isoaspartate'>IAS</scene>
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=IAS:ASPARTYL+GROUP'>IAS</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17] </span>
+or leave the SCENE parameter empty for the default display.
-|GENE=
+-->
-|DOMAIN=
+{{STRUCTURE_1at6|  PDB=1at6  |  SCENE=  }}
-|RELATEDENTRY=
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1at6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1at6 OCA], [http://www.ebi.ac.uk/pdbsum/1at6 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1at6 RCSB]</span>
-}}
 '''HEN EGG WHITE LYSOZYME WITH A ISOASPARTATE RESIDUE'''
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 [[Category: Noguchi, S.]]
 [[Category: Satow, Y.]]
-[[Category: hydrolase]]
+[[Category: Hydrolase]]
-[[Category: isoaspartate]]
+[[Category: Isoaspartate]]
-[[Category: o-glycosyl hydrolase]]
+[[Category: O-glycosyl hydrolase]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 10:40:00 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 18:47:01 2008''