1as6: Difference between revisions

Revision as of 10:38, 2 May 2008

STRUCTURE OF NITRITE BOUND TO OXIDIZED ALCALIGENES FAECALIS NITRITE REDUCTASE AT CRYO TEMPERATURE

OverviewOverview

The structures of oxidized, reduced, nitrite-soaked oxidized and nitrite-soaked reduced nitrite reductase from Alcaligenes faecalis have been determined at 1.8-2.0 A resolution using data collected at -160 degrees C. The active site at cryogenic temperature, as at room temperature, contains a tetrahedral type II copper site liganded by three histidines and a water molecule. The solvent site is empty when crystals are reduced with ascorbate. A fully occupied oxygen-coordinate nitrite occupies the solvent site in crystals soaked in nitrite. Ascorbate-reduced crystals soaked in a glycerol-methanol solution and nitrite at -40 degrees C remain colorless at -160 degrees C but turn amber-brown when warmed, suggesting that NO is released. Nitrite is found at one-half occupancy. Five new solvent sites in the oxidized nitrite bound form exhibit defined but different occupancies in the other three forms. These results support a previously proposed mechanism by which nitrite is bound primarily by a single oxygen atom that is protonable, and after reduction and cleavage of that N-O bond, NO is released leaving the oxygen atom bound to the Cu site as hydroxide or water.

About this StructureAbout this Structure

1AS6 is a Single protein structure of sequence from Alcaligenes faecalis. Full crystallographic information is available from OCA.

ReferenceReference

Structure of nitrite bound to copper-containing nitrite reductase from Alcaligenes faecalis. Mechanistic implications., Murphy ME, Turley S, Adman ET, J Biol Chem. 1997 Nov 7;272(45):28455-60. PMID:9353305 Page seeded by OCA on Fri May 2 10:38:26 2008

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OCA

@@ Line 1: / Line 1: @@
 [[Image:1as6.jpg|left|200px]]
- {{Structure
+<!--
-|PDB= 1as6 |SIZE=350|CAPTION= <scene name='initialview01'>1as6</scene>, resolution 1.8&Aring;
+The line below this paragraph, containing "STRUCTURE_1as6", creates the "Structure Box" on the page.
-|SITE= <scene name='pdbsite=CU1:Cu+Site'>CU1</scene>, <scene name='pdbsite=CU2:Cu+Site'>CU2</scene>, <scene name='pdbsite=CU3:Cu+Site'>CU3</scene>, <scene name='pdbsite=CU4:Cu+Site'>CU4</scene>, <scene name='pdbsite=CU5:Cu+Site'>CU5</scene> and <scene name='pdbsite=CU6:Cu+Site'>CU6</scene>
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=NO2:NITRITE+ION'>NO2</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Nitrite_reductase_(NO-forming) Nitrite reductase (NO-forming)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.7.2.1 1.7.2.1] </span>
+or leave the SCENE parameter empty for the default display.
-|GENE=
+-->
-|DOMAIN=
+{{STRUCTURE_1as6|  PDB=1as6  |  SCENE=  }}
-|RELATEDENTRY=
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1as6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1as6 OCA], [http://www.ebi.ac.uk/pdbsum/1as6 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1as6 RCSB]</span>
-}}
 '''STRUCTURE OF NITRITE BOUND TO OXIDIZED ALCALIGENES FAECALIS NITRITE REDUCTASE AT CRYO TEMPERATURE'''
@@ Line 24: / Line 21: @@
 Structure of nitrite bound to copper-containing nitrite reductase from Alcaligenes faecalis. Mechanistic implications., Murphy ME, Turley S, Adman ET, J Biol Chem. 1997 Nov 7;272(45):28455-60. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9353305 9353305]
 [[Category: Alcaligenes faecalis]]
-[[Category: Nitrite reductase (NO-forming)]]
 [[Category: Single protein]]
 [[Category: Adman, E T.]]
 [[Category: Murphy, M E.P.]]
 [[Category: Turley, S.]]
-[[Category: copper]]
+[[Category: Copper]]
-[[Category: denitrification]]
+[[Category: Denitrification]]
-[[Category: nitrite]]
+[[Category: Nitrite]]
-[[Category: oxidoreductase]]
+[[Category: Oxidoreductase]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 10:38:26 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 18:46:34 2008''