1ao6: Difference between revisions
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'''CRYSTAL STRUCTURE OF HUMAN SERUM ALBUMIN''' | '''CRYSTAL STRUCTURE OF HUMAN SERUM ALBUMIN''' | ||
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[[Category: Noda, M.]] | [[Category: Noda, M.]] | ||
[[Category: Sugio, S.]] | [[Category: Sugio, S.]] | ||
[[Category: | [[Category: Albumin]] | ||
[[Category: | [[Category: Carrier protein]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 10:30:11 2008'' | |||
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Revision as of 10:30, 2 May 2008
CRYSTAL STRUCTURE OF HUMAN SERUM ALBUMIN
OverviewOverview
A new triclinic crystal form of human serum albumin (HSA), derived either from pool plasma (pHSA) or from a Pichia pastoris expression system (rHSA), was obtained from polyethylene glycol 4000 solution. Three-dimensional structures of pHSA and rHSA were determined at 2.5 A resolution from the new triclinic crystal form by molecular replacement, using atomic coordinates derived from a multiple isomorphous replacement work with a known tetragonal crystal form. The structures of pHSA and rHSA are virtually identical, with an r.m. s. deviation of 0.24 A for all Calpha atoms. The two HSA molecules involved in the asymmetric unit are related by a strict local twofold symmetry such that the Calpha atoms of the two molecules can be superimposed with an r.m.s. deviation of 0.28 A in pHSA. Cys34 is the only cysteine with a free sulfhydryl group which does not participate in a disulfide linkage with any external ligand. Domains II and III both have a pocket formed mostly of hydrophobic and positively charged residues and in which a very wide range of compounds may be accommodated. Three tentative binding sites for long-chain fatty acids, each with different surroundings, are located at the surface of each domain.
About this StructureAbout this Structure
1AO6 is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
ReferenceReference
Crystal structure of human serum albumin at 2.5 A resolution., Sugio S, Kashima A, Mochizuki S, Noda M, Kobayashi K, Protein Eng. 1999 Jun;12(6):439-46. PMID:10388840 Page seeded by OCA on Fri May 2 10:30:11 2008