1ag1: Difference between revisions

Revision as of 10:13, 2 May 2008

MONOHYDROGEN PHOSPHATE BINDING TO TRYPANOSOMAL TRIOSEPHOSPHATE ISOMERASE

OverviewOverview

The three-dimensional structure of triosephosphate isomerase complexed with the competitive inhibitor SO-4(2) was determined by X-ray crystallography to a resolution of 0.24 nm. A comparison with the native crystal structure, where SO-4(2) is bound, revealed five changes: (a) a 0.10-nm shift of the anion-binding site; (b) a further closing of the flexible loop of the enzyme; (c) a 'swinging in' of the side chain of the catalytic Glu, that is chi 1 changes from (+) to (-) synclinal; (d) an altered water structure; (e) a disappearance of the conformational heterogeneity at the C-terminus of strand beta 7. Some of these changes may be related to the different hydrogen-bond pattern about the two different anions. However, the distance of 0.10 nm between the sulphur and phosphorus positions is unexpected and remains intriguing.

About this StructureAbout this Structure

1AG1 is a Single protein structure of sequence from Trypanosoma brucei. Full crystallographic information is available from OCA.

ReferenceReference

Anion binding at the active site of trypanosomal triosephosphate isomerase. Monohydrogen phosphate does not mimic sulphate., Verlinde CL, Noble ME, Kalk KH, Groendijk H, Wierenga RK, Hol WG, Eur J Biochem. 1991 May 23;198(1):53-7. PMID:2040290 Page seeded by OCA on Fri May 2 10:13:16 2008

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OCA

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 [[Image:1ag1.jpg|left|200px]]
- {{Structure
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+The line below this paragraph, containing "STRUCTURE_1ag1", creates the "Structure Box" on the page.
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-|LIGAND= <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>
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-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Triose-phosphate_isomerase Triose-phosphate isomerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.3.1.1 5.3.1.1] </span>
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-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ag1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ag1 OCA], [http://www.ebi.ac.uk/pdbsum/1ag1 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1ag1 RCSB]</span>
-}}
 '''MONOHYDROGEN PHOSPHATE BINDING TO TRYPANOSOMAL TRIOSEPHOSPHATE ISOMERASE'''
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 [[Category: Hol, W G.J.]]
 [[Category: Verlinde, C L.M J.]]
-[[Category: isomerase (intramolecular oxidoreductase)]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 10:13:16 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 18:39:23 2008''