1a35: Difference between revisions

Revision as of 09:44, 2 May 2008

HUMAN TOPOISOMERASE I/DNA COMPLEX

OverviewOverview

Topoisomerases I promote the relaxation of DNA superhelical tension by introducing a transient single-stranded break in duplex DNA and are vital for the processes of replication, transcription, and recombination. The crystal structures at 2.1 and 2.5 angstrom resolution of reconstituted human topoisomerase I comprising the core and carboxyl-terminal domains in covalent and noncovalent complexes with 22-base pair DNA duplexes reveal an enzyme that "clamps" around essentially B-form DNA. The core domain and the first eight residues of the carboxyl-terminal domain of the enzyme, including the active-site nucleophile tyrosine-723, share significant structural similarity with the bacteriophage family of DNA integrases. A binding mode for the anticancer drug camptothecin is proposed on the basis of chemical and biochemical information combined with these three-dimensional structures of topoisomerase I-DNA complexes.

About this StructureAbout this Structure

1A35 is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

ReferenceReference

Crystal structures of human topoisomerase I in covalent and noncovalent complexes with DNA., Redinbo MR, Stewart L, Kuhn P, Champoux JJ, Hol WG, Science. 1998 Mar 6;279(5356):1504-13. PMID:9488644 Page seeded by OCA on Fri May 2 09:44:25 2008

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OCA

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 [[Image:1a35.jpg|left|200px]]
- {{Structure
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+The line below this paragraph, containing "STRUCTURE_1a35", creates the "Structure Box" on the page.
-|SITE=
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
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+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/DNA_topoisomerase DNA topoisomerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.99.1.2 5.99.1.2] </span>
+or leave the SCENE parameter empty for the default display.
-|GENE=
+-->
-|DOMAIN=
+{{STRUCTURE_1a35|  PDB=1a35  |  SCENE=  }}
-|RELATEDENTRY=
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1a35 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1a35 OCA], [http://www.ebi.ac.uk/pdbsum/1a35 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1a35 RCSB]</span>
-}}
 '''HUMAN TOPOISOMERASE I/DNA COMPLEX'''
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 [[Category: Redinbo, M R.]]
 [[Category: Stewart, L.]]
-[[Category: dna]]
+[[Category: Dna]]
-[[Category: topoisomerase i]]
+[[Category: Topoisomerase i]]
-[[Category: topoisomerase i/dna)]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 09:44:25 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 18:32:14 2008''