197d: Difference between revisions

Revision as of 09:35, 2 May 2008

ORTHORHOMBIC CRYSTAL STRUCTURE OF THE A-DNA OCTAMER D(GTACGTAC). COMPARISON WITH THE TETRAGONAL STRUCTURE

OverviewOverview

The X-ray crystal structure of the double-helical A-DNA octanucleotide d(GTACGTAC) has been solved by molecular replacement and refined to a resolution of 0.219 nm. The final R-factor is equal to 16.1% for 1516 observed reflections with F > 4 sigma(F). The sequence crystallizes as an A-DNA-type double helix in the orthorhombic space group P2(1)2(1)2, with one duplex molecule solvated by 66 water molecules in the asymmetric unit. Cell parameters are a = 3.860 nm, b = 5.082 nm, c = 2.174 nm. It is the first time that such a crystal form has been observed. This orthorhombic structure has been compared with the tetragonal structure of the same oligonucleotide. It adopts a bent structure with an unusual packing between symmetry-related molecules.

About this StructureAbout this Structure

Full crystallographic information is available from OCA.

ReferenceReference

Orthorhombic crystal structure of the A-DNA octamer d(GTACGTAC). Comparison with the tetragonal structure., Langlois d'Estaintot B, Dautant A, Courseille C, Precigoux G, Eur J Biochem. 1993 Apr 15;213(2):673-82. PMID:8477741 Page seeded by OCA on Fri May 2 09:35:43 2008

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OCA

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 [[Image:197d.gif|left|200px]]
- {{Structure
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 '''ORTHORHOMBIC CRYSTAL STRUCTURE OF THE A-DNA OCTAMER D(GTACGTAC). COMPARISON WITH THE TETRAGONAL STRUCTURE'''
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 ==About this Structure==
-D is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=197D OCA].
+Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=197D OCA].
 ==Reference==
 Orthorhombic crystal structure of the A-DNA octamer d(GTACGTAC). Comparison with the tetragonal structure., Langlois d'Estaintot B, Dautant A, Courseille C, Precigoux G, Eur J Biochem. 1993 Apr 15;213(2):673-82. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/8477741 8477741]
-[[Category: Protein complex]]
 [[Category: Courseille, C.]]
 [[Category: Dautant, A.]]
 [[Category: Estaintot, B Langlois D.]]
 [[Category: Precigoux, G.]]
-[[Category: a-dna]]
+[[Category: A-dna]]
-[[Category: double helix]]
+[[Category: Double helix]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 09:35:43 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 18:30:09 2008''