4z44: Difference between revisions

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'''Unreleased structure'''


The entry 4z44 is ON HOLD
==F454K Mutant of Tryptophan 7-halogenase PrnA==
<StructureSection load='4z44' size='340' side='right' caption='[[4z44]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[4z44]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4Z44 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4Z44 FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4z43|4z43]]</td></tr>
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Tryptophan_7-halogenase Tryptophan 7-halogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.14.19.9 1.14.19.9] </span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4z44 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4z44 OCA], [http://pdbe.org/4z44 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4z44 RCSB], [http://www.ebi.ac.uk/pdbsum/4z44 PDBsum]</span></td></tr>
</table>
== Function ==
[[http://www.uniprot.org/uniprot/PRNA_PSEFL PRNA_PSEFL]] Involved in the biosynthesis of the antifungal antibiotic pyrrolnitrin. Catalyze the chlorination of tryptophan (Trp) at C7 position to yield 7-chloro-L-tryptophan (7-CLT). The reaction between FADH2, Cl-, and O2 generates the powerful oxidant HOCl, which is presumed to carry out the chlorination reaction. The reaction of HOCl with the active site Lys-79 generates a lysine chloramine, which plays a key role in directing regiospecific chlorination of substrate in this important class of biosynthetic enzymes. It is also able to use bromide ions to generate monobrominated Trp.<ref>PMID:10941070</ref> <ref>PMID:9172332</ref> <ref>PMID:9537395</ref> 


Authors: Shepherd, S.A., Karthikeyan, C., Latham, J., Struck, A.-W., Thompson, M.L., Menon, B., Levy, C.W., Leys, D., Micklefield, J.
==See Also==
 
*[[Ribosome inactivating protein|Ribosome inactivating protein]]
Description: F454K Mutant of Tryptophan 7-halogenase PrnA
== References ==
[[Category: Unreleased Structures]]
<references/>
[[Category: Thompson, M.L]]
__TOC__
[[Category: Micklefield, J]]
</StructureSection>
[[Category: Shepherd, S.A]]
[[Category: Tryptophan 7-halogenase]]
[[Category: Karthikeyan, C]]
[[Category: Latham, J]]
[[Category: Levy, C W]]
[[Category: Leys, D]]
[[Category: Leys, D]]
[[Category: Menon, B]]
[[Category: Menon, B]]
[[Category: Latham, J]]
[[Category: Micklefield, J]]
[[Category: Karthikeyan, C]]
[[Category: Shepherd, S A]]
[[Category: Levy, C.W]]
[[Category: Struck, A W]]
[[Category: Struck, A.-W]]
[[Category: Thompson, M L]]
[[Category: Oxidoreductase]]

Revision as of 14:20, 12 May 2016

F454K Mutant of Tryptophan 7-halogenase PrnAF454K Mutant of Tryptophan 7-halogenase PrnA

Structural highlights

4z44 is a 1 chain structure. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:, , ,
Activity:Tryptophan 7-halogenase, with EC number 1.14.19.9
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum

Function

[PRNA_PSEFL] Involved in the biosynthesis of the antifungal antibiotic pyrrolnitrin. Catalyze the chlorination of tryptophan (Trp) at C7 position to yield 7-chloro-L-tryptophan (7-CLT). The reaction between FADH2, Cl-, and O2 generates the powerful oxidant HOCl, which is presumed to carry out the chlorination reaction. The reaction of HOCl with the active site Lys-79 generates a lysine chloramine, which plays a key role in directing regiospecific chlorination of substrate in this important class of biosynthetic enzymes. It is also able to use bromide ions to generate monobrominated Trp.[1] [2] [3]

See Also

References

  1. ↑ Keller S, Wage T, Hohaus K, Holzer M, Eichhorn E, van Pee KH. Purification and Partial Characterization of Tryptophan 7-Halogenase (PrnA) from Pseudomonas fluorescens This work was supported by the Deutsche Forschungsgemeinschaft (DFG) through the Graduiertenkolleg "Struktur-Eigenschafts-Beziehungen bei Heterocyclen", the Environment and Climate Research and Technology Development Programme of the European Union, the Sachsische Staatsministerium fur Umwelt und Landesentwicklung, the Max-Buchner-Stiftung, and the Fonds der Chemischen Industrie. Samples of P. fluorescens BL915DeltaORF1-4 with pPEH14(prnA) and pPEH14(prnC) were obtained from Dr. J. M. Ligon, Novartis Agribusiness Biotechnology Research, Inc., Research Triangle, NC (USA) and NADH oxidase (from Thermus thermiphilus) from Prof. Helmut Erdmann, Fachhochschule Flensburg (Germany). Angew Chem Int Ed Engl. 2000 Jul 3;39(13):2300-2302. PMID:10941070
  2. ↑ Hammer PE, Hill DS, Lam ST, Van Pee KH, Ligon JM. Four genes from Pseudomonas fluorescens that encode the biosynthesis of pyrrolnitrin. Appl Environ Microbiol. 1997 Jun;63(6):2147-54. PMID:9172332
  3. ↑ Kirner S, Hammer PE, Hill DS, Altmann A, Fischer I, Weislo LJ, Lanahan M, van Pee KH, Ligon JM. Functions encoded by pyrrolnitrin biosynthetic genes from Pseudomonas fluorescens. J Bacteriol. 1998 Apr;180(7):1939-43. PMID:9537395

4z44, resolution 2.20Å

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