12gs: Difference between revisions

Revision as of 09:28, 2 May 2008

GLUTATHIONE S-TRANSFERASE COMPLEXED WITH S-NONYL-GLUTATHIONE

OverviewOverview

Glutathione S -transferases (GSTs) play a pivotal role in the detoxification of foreign chemicals and toxic metabolites. They were originally termed ligandins because of their ability to bind large molecules (molecular masses >400 Da), possibly for storage and transport roles. The location of the ligandin site in mammalian GSTs is still uncertain despite numerous studies in recent years. Here we show by X-ray crystallography that the ligandin binding site in human pi class GST P1-1 occupies part of one of the substrate binding sites. This work has been extended to the determination of a number of enzyme complex crystal structures which show that very large ligands are readily accommodated into this substrate binding site and in all, but one case, causes no significant movement of protein side-chains. Some of these molecules make use of a hitherto undescribed binding site located in a surface pocket of the enzyme. This site is conserved in most, but not all, classes of GSTs suggesting it may play an important functional role.

About this StructureAbout this Structure

12GS is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

ReferenceReference

The ligandin (non-substrate) binding site of human Pi class glutathione transferase is located in the electrophile binding site (H-site)., Oakley AJ, Lo Bello M, Nuccetelli M, Mazzetti AP, Parker MW, J Mol Biol. 1999 Aug 27;291(4):913-26. PMID:10452896 Page seeded by OCA on Fri May 2 09:28:38 2008

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OCA

@@ Line 1: / Line 1: @@
 [[Image:12gs.gif|left|200px]]
- {{Structure
+<!--
-|PDB= 12gs |SIZE=350|CAPTION= <scene name='initialview01'>12gs</scene>, resolution 2.10&Aring;
+The line below this paragraph, containing "STRUCTURE_12gs", creates the "Structure Box" on the page.
-|SITE=
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=GT9:S-NONYL-CYSTEINE'>GT9</scene>, <scene name='pdbligand=MES:2-(N-MORPHOLINO)-ETHANESULFONIC+ACID'>MES</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Glutathione_transferase Glutathione transferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.18 2.5.1.18] </span>
+or leave the SCENE parameter empty for the default display.
-|GENE= GSTP1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])
+-->
-|DOMAIN=
+{{STRUCTURE_12gs|  PDB=12gs  |  SCENE=  }}
-|RELATEDENTRY=
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=12gs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=12gs OCA], [http://www.ebi.ac.uk/pdbsum/12gs PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=12gs RCSB]</span>
-}}
 '''GLUTATHIONE S-TRANSFERASE COMPLEXED WITH S-NONYL-GLUTATHIONE'''
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 [[Category: Oakley, A J.]]
 [[Category: Parker, M W.]]
-[[Category: complex (transferase/nonyl-glutathione)]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May  2 09:28:38 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 18:27:34 2008''