4zf7: Difference between revisions
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==Crystal structure of ferret interleukin-2== | ==Crystal structure of ferret interleukin-2== | ||
<StructureSection load='4zf7' size='340' side='right' caption='[[4zf7]], [[Resolution|resolution]] 1.89Å' scene=''> | <StructureSection load='4zf7' size='340' side='right' caption='[[4zf7]], [[Resolution|resolution]] 1.89Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[4zf7]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ZF7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ZF7 FirstGlance]. <br> | <table><tr><td colspan='2'>[[4zf7]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/European_domestic_ferret European domestic ferret]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ZF7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ZF7 FirstGlance]. <br> | ||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=1PE:PENTAETHYLENE+GLYCOL'>1PE</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=1PE:PENTAETHYLENE+GLYCOL'>1PE</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4zf7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4zf7 OCA], [http://pdbe.org/4zf7 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4zf7 RCSB], [http://www.ebi.ac.uk/pdbsum/4zf7 PDBsum]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4zf7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4zf7 OCA], [http://pdbe.org/4zf7 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4zf7 RCSB], [http://www.ebi.ac.uk/pdbsum/4zf7 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4zf7 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
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__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: European domestic ferret]] | |||
[[Category: Adams, T E]] | [[Category: Adams, T E]] | ||
[[Category: McKinstry, W J]] | [[Category: McKinstry, W J]] | ||
Revision as of 09:15, 1 November 2017
Crystal structure of ferret interleukin-2Crystal structure of ferret interleukin-2
Structural highlights
Publication Abstract from PubMedWhile the ferret is a valuable animal model for a number of human viral infections, such as influenza, Hendra and Nipah, evaluating the cellular immune response following infection has been hampered by the lack of a number of species-specific immunological reagents. Interleukin 2 (IL-2) is one such key cytokine. Ferret recombinant IL-2 incorporating a C-terminal histidine tag was expressed and purified and the three-dimensional structure solved and refined at 1.89 A by X-ray crystallography, which represents the highest resolution and first non-human IL-2 structure. While ferret IL-2 displays the classic cytokine fold of the four-helix bundle structure, conformational flexibility was observed at the second helix and its neighbouring region in the bundle, which may result in the disruption of the spatial arrangement of residues involved in receptor binding interactions, implicating subtle differences between ferret and human IL-2 when initiating biological functions. Ferret recombinant IL-2 stimulated the proliferation of ferret lymph node cells and induced the expression of mRNA for IFN-gamma and Granzyme A. Structural and functional characterisation of ferret interleukin-2.,Ren B, McKinstry WJ, Pham T, Newman J, Layton DS, Bean AG, Chen Z, Laurie KL, Borg K, Barr IG, Adams TE Dev Comp Immunol. 2015 Oct 22;55:32-38. doi: 10.1016/j.dci.2015.10.007. PMID:26472619[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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