CCA-adding enzyme: Difference between revisions

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'''CCA-adding enzyme''' (CAE) builds or repairs the trinucleotice CCA at the 3’ end of tRNA.  CAE has one active site which is capable of binding CTP and ATP in a specific sequence.  In the bacteria Aquifex aeolicus, CAE consists of 2 subunits.  One specific for CTP and the other for ATP.  CAE builds the CCA without a genetic template.<ref>PMID:15295590</ref>
'''CCA-adding enzyme''' (CAE) builds or repairs the trinucleotice CCA at the 3’ end of tRNA.  CAE has one active site which is capable of binding CTP and ATP in a specific sequence.  In the bacteria Aquifex aeolicus, CAE consists of 2 subunits.  One specific for CTP and the other for ATP.  CAE builds the CCA without a genetic template.<ref>PMID:15295590</ref>
*<scene name='55/551188/Cv/2'>CCA-adding enzyme complex with tRNA, CTP, ethanediol and Ca+2 ion</scene> (PDB entry [[3ovs]]).
*<scene name='55/551188/Cv/3'>Ca+2 binding site of CCA-adding enzyme</scene> (PDB entry [[3ovs]]).<ref>PMID:21071662</ref>
*<scene name='55/551188/Cv/3'>Ca+2 binding site of CCA-adding enzyme</scene> (PDB entry [[3ovs]]).<ref>PMID:21071662</ref>
</StructureSection>
</StructureSection>

Revision as of 14:17, 29 November 2015


CCA-adding enzyme (CAE) builds or repairs the trinucleotice CCA at the 3’ end of tRNA. CAE has one active site which is capable of binding CTP and ATP in a specific sequence. In the bacteria Aquifex aeolicus, CAE consists of 2 subunits. One specific for CTP and the other for ATP. CAE builds the CCA without a genetic template.[1]

Structure of CCA-adding enzyme complex with tRNA 34-mer, CTP, sulfate, ethanediol and Ca+2 ion (green) (PDB entry 3ovs)

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3D structures of CCA-adding enzyme3D structures of CCA-adding enzyme

Updated on 29-November-2015

ReferencesReferences

  1. ↑ Xiong Y, Steitz TA. Mechanism of transfer RNA maturation by CCA-adding enzyme without using an oligonucleotide template. Nature. 2004 Aug 5;430(7000):640-5. PMID:15295590 doi:http://dx.doi.org/10.1038/nature02711
  2. ↑ Pan B, Xiong Y, Steitz TA. How the CCA-adding enzyme selects adenine over cytosine at position 76 of tRNA. Science. 2010 Nov 12;330(6006):937-40. PMID:21071662 doi:10.1126/science.1194985

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