5a96: Difference between revisions
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==Crystal structure of Lymantria dispar CPV14 polyhedra== | ==Crystal structure of Lymantria dispar CPV14 polyhedra== | ||
<StructureSection load='5a96' size='340' side='right' caption='[[5a96]], [[Resolution|resolution]] 1.91Å' scene=''> | <StructureSection load='5a96' size='340' side='right'caption='[[5a96]], [[Resolution|resolution]] 1.91Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[5a96]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5A96 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5A96 FirstGlance]. <br> | <table><tr><td colspan='2'>[[5a96]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Lymantria_dispar_cypovirus_14 Lymantria dispar cypovirus 14]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5A96 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5A96 FirstGlance]. <br> | ||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene></td></tr> | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene></td></tr> | ||
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5a8s|5a8s]], [[5a8t|5a8t]], [[5a8u|5a8u]], [[5a8v|5a8v]], [[5a98|5a98]], [[5a99|5a99]], [[5a9a|5a9a]], [[5a9b|5a9b]], [[5a9c|5a9c]], [[5a9p|5a9p]]</td></tr> | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5a8s|5a8s]], [[5a8t|5a8t]], [[5a8u|5a8u]], [[5a8v|5a8v]], [[5a98|5a98]], [[5a99|5a99]], [[5a9a|5a9a]], [[5a9b|5a9b]], [[5a9c|5a9c]], [[5a9p|5a9p]]</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5a96 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5a96 OCA], [http://pdbe.org/5a96 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5a96 RCSB], [http://www.ebi.ac.uk/pdbsum/5a96 PDBsum]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5a96 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5a96 OCA], [http://pdbe.org/5a96 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5a96 RCSB], [http://www.ebi.ac.uk/pdbsum/5a96 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5a96 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
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__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | |||
[[Category: Lymantria dispar cypovirus 14]] | |||
[[Category: Axford, D]] | [[Category: Axford, D]] | ||
[[Category: Evans, G]] | [[Category: Evans, G]] | ||
Revision as of 11:05, 26 June 2019
Crystal structure of Lymantria dispar CPV14 polyhedraCrystal structure of Lymantria dispar CPV14 polyhedra
Structural highlights
Publication Abstract from PubMedPolyhedra represent an ancient system used by a number of insect viruses to protect virions during long periods of environmental exposure. We present high resolution crystal structures of polyhedra for seven previously uncharacterised types of cypoviruses, four using ab initio selenomethionine phasing (two of these required over 100 selenomethionine crystals each). Approximately 80% of residues are structurally equivalent between all polyhedrins (pairwise rmsd 1.5A), whilst pairwise sequence identities, based on structural alignment, are as little as 12%. These structures illustrate the effect of 400million years of evolution on a system where the crystal lattice is the functionally conserved feature in the face of massive sequence variability. The conservation of crystal contacts is maintained across most of the molecular surface, except for a dispensable virus recognition domain. By spreading the contacts over so much of the protein surface the lattice remains robust in the face of many individual changes. Overall these unusual structural constraints seem to have skewed the molecule's evolution so that surface residues are almost as conserved as the internal residues. Polyhedra structures and the evolution of the insect viruses.,Ji X, Axford D, Owen R, Evans G, Ginn HM, Sutton G, Stuart DI J Struct Biol. 2015 Aug 18. pii: S1047-8477(15)30047-2. doi:, 10.1016/j.jsb.2015.08.009. PMID:26291392[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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