2npj: Difference between revisions
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==An unusual twin-His arrangement in the pore of ammonia channels is essential for substrate conductance== | ==An unusual twin-His arrangement in the pore of ammonia channels is essential for substrate conductance== | ||
<StructureSection load='2npj' size='340' side='right' caption='[[2npj]], [[Resolution|resolution]] 2.00Å' scene=''> | <StructureSection load='2npj' size='340' side='right' caption='[[2npj]], [[Resolution|resolution]] 2.00Å' scene=''> | ||
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1xqf|1xqf]], [[1xqe|1xqe]], [[1u7g|1u7g]], [[2nmr|2nmr]], [[2nop|2nop]], [[2now|2now]], [[2npc|2npc]], [[2npd|2npd]], [[2npe|2npe]], [[2npg|2npg]], [[2npk|2npk]]</td></tr> | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1xqf|1xqf]], [[1xqe|1xqe]], [[1u7g|1u7g]], [[2nmr|2nmr]], [[2nop|2nop]], [[2now|2now]], [[2npc|2npc]], [[2npd|2npd]], [[2npe|2npe]], [[2npg|2npg]], [[2npk|2npk]]</td></tr> | ||
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">amtB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895])</td></tr> | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">amtB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895])</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2npj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2npj OCA], [http://pdbe.org/2npj PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2npj RCSB], [http://www.ebi.ac.uk/pdbsum/2npj PDBsum]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2npj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2npj OCA], [http://pdbe.org/2npj PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2npj RCSB], [http://www.ebi.ac.uk/pdbsum/2npj PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2npj ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Function == | == Function == | ||
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<text>to colour the structure by Evolutionary Conservation</text> | <text>to colour the structure by Evolutionary Conservation</text> | ||
</jmolCheckbox> | </jmolCheckbox> | ||
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/ | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2npj ConSurf]. | ||
<div style="clear:both"></div> | <div style="clear:both"></div> | ||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
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</div> | </div> | ||
<div class="pdbe-citations 2npj" style="background-color:#fffaf0;"></div> | <div class="pdbe-citations 2npj" style="background-color:#fffaf0;"></div> | ||
== References == | == References == | ||
<references/> | <references/> | ||
Revision as of 11:39, 18 October 2017
An unusual twin-His arrangement in the pore of ammonia channels is essential for substrate conductanceAn unusual twin-His arrangement in the pore of ammonia channels is essential for substrate conductance
Structural highlights
Function[AMTB_ECOLI] Involved in the uptake of ammonia. Evolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMedAmt proteins constitute a class of ubiquitous integral membrane proteins that mediate movement of ammonium across cell membranes. They are homotrimers, in which each subunit contains a narrow pore through which substrate transport occurs. Two conserved histidine residues in the pore have been proposed to be necessary for ammonia conductance. By analyzing 14 engineered polar and non-polar variants of these histidines, in Escherichia coli AmtB, we show that both histidines are absolutely required for optimum substrate conductance. Crystal structures of variants confirm that substitution of the histidine residues does not affect AmtB structure. In a subgroup of Amt proteins, found only in fungi, one of the histidines is replaced by glutamate. The equivalent substitution in E. coli AmtB is partially active, and the structure of this variant suggests that the glutamate side chain can make similar interactions to those made by histidine. An unusual twin-his arrangement in the pore of ammonia channels is essential for substrate conductance.,Javelle A, Lupo D, Zheng L, Li XD, Winkler FK, Merrick M J Biol Chem. 2006 Dec 22;281(51):39492-8. Epub 2006 Oct 12. PMID:17040913[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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