1d29: Difference between revisions
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==CRYSTAL STRUCTURE ANALYSIS OF THE B-DNA DODECAMER CGTGAATTCACG== | ==CRYSTAL STRUCTURE ANALYSIS OF THE B-DNA DODECAMER CGTGAATTCACG== | ||
<StructureSection load='1d29' size='340' side='right' caption='[[1d29]], [[Resolution|resolution]] 2.50Å' scene=''> | <StructureSection load='1d29' size='340' side='right' caption='[[1d29]], [[Resolution|resolution]] 2.50Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[1d29]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1D29 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1D29 FirstGlance]. <br> | <table><tr><td colspan='2'>[[1d29]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1D29 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1D29 FirstGlance]. <br> | ||
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1d29 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1d29 OCA], [http://pdbe.org/1d29 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1d29 RCSB], [http://www.ebi.ac.uk/pdbsum/1d29 PDBsum]</span></td></tr> | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1d29 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1d29 OCA], [http://pdbe.org/1d29 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1d29 RCSB], [http://www.ebi.ac.uk/pdbsum/1d29 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1d29 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
Revision as of 14:12, 24 August 2017
CRYSTAL STRUCTURE ANALYSIS OF THE B-DNA DODECAMER CGTGAATTCACGCRYSTAL STRUCTURE ANALYSIS OF THE B-DNA DODECAMER CGTGAATTCACG
Structural highlights
Publication Abstract from PubMedThe crystal structure of the DNA dodecamer C-G-T-G-A-A-T-T-C-A-C-G has been determined at a resolution of 2.5 A, with a final R factor of 15.8% for 1475 nonzero reflections measured at 0 degrees C. The structure is isomorphous with that of the Drew dodecamer, with the space group P2(1)2(1)2(1) and cell dimensions of a = 24.94 A, b = 40.78 A, and c = 66.13 A. The asymmetric unit contains all 12 base pairs of the B-DNA double helix and 36 water molecules. The structure of C-G-T-G-A-A-T-T-C-A-C-G is very similar to that of C-G-C-G-A-A-T-T-C-G-C-G, with no major alterations in helix parameters. Water peaks in the refined structure appear to represent a selection of peaks that were observed in the Drew dodecamer. The minor-groove spine of hydration at 2.5 A is fragmentary, but as Narendra et al. (1991) [Biochemistry (following paper in this issue)] have observed, lowering the temperature leads to a more complete representation of the spine. Crystal structure analysis of the B-DNA dodecamer CGTGAATTCACG.,Larsen TA, Kopka ML, Dickerson RE Biochemistry. 1991 May 7;30(18):4443-9. PMID:1850624[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References |
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