1mjh: Difference between revisions

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Revision as of 06:56, 26 March 2008

File:1mjh.gif

, resolution 1.7Å

Ligands:

,

Gene:

MJ0577 (Methanocaldococcus jannaschii)

Domains:

USP_Like

Resources:

FirstGlance, OCA, PDBsum, JenaLib, RCSB

Coordinates:

save as pdb, mmCIF, xml

STRUCTURE-BASED ASSIGNMENT OF THE BIOCHEMICAL FUNCTION OF HYPOTHETICAL PROTEIN MJ0577: A TEST CASE OF STRUCTURAL GENOMICS

OverviewOverview

Many small bacterial, archaebacterial, and eukaryotic genomes have been sequenced, and the larger eukaryotic genomes are predicted to be completely sequenced within the next decade. In all genomes sequenced to date, a large portion of these organisms' predicted protein coding regions encode polypeptides of unknown biochemical, biophysical, and/or cellular functions. Three-dimensional structures of these proteins may suggest biochemical or biophysical functions. Here we report the crystal structure of one such protein, MJ0577, from a hyperthermophile, Methanococcus jannaschii, at 1.7-A resolution. The structure contains a bound ATP, suggesting MJ0577 is an ATPase or an ATP-mediated molecular switch, which we confirm by biochemical experiments. Furthermore, the structure reveals different ATP binding motifs that are shared among many homologous hypothetical proteins in this family. This result indicates that structure-based assignment of molecular function is a viable approach for the large-scale biochemical assignment of proteins and for discovering new motifs, a basic premise of structural genomics.

About this StructureAbout this Structure

1MJH is a Single protein structure of sequence from Methanocaldococcus jannaschii. Full crystallographic information is available from OCA.

ReferenceReference

Structure-based assignment of the biochemical function of a hypothetical protein: a test case of structural genomics., Zarembinski TI, Hung LW, Mueller-Dieckmann HJ, Kim KK, Yokota H, Kim R, Kim SH, Proc Natl Acad Sci U S A. 1998 Dec 22;95(26):15189-93. PMID:9860944

Page seeded by OCA on Wed Mar 26 05:56:56 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 4: / Line 4: @@
 |PDB= 1mjh |SIZE=350|CAPTION= <scene name='initialview01'>1mjh</scene>, resolution 1.7&Aring;
 |SITE=
-|LIGAND= <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene> and <scene name='pdbligand=ATP:ADENOSINE-5&#39;-TRIPHOSPHATE'>ATP</scene>
+|LIGAND= <scene name='pdbligand=ATP:ADENOSINE-5&#39;-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>
 |ACTIVITY=
 |GENE= MJ0577 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2190 Methanocaldococcus jannaschii])
+|DOMAIN=<span class='plainlinks'>[http://www.ncbi.nlm.nih.gov/Structure/cdd/cddsrv.cgi?uid=cd00293 USP_Like]</span>
+|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1mjh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mjh OCA], [http://www.ebi.ac.uk/pdbsum/1mjh PDBsum], [http://www.fli-leibniz.de/cgi-bin/ImgLib.pl?CODE=1kfv JenaLib], [http://www.rcsb.org/pdb/explore.do?structureId=1mjh RCSB]</span>
 }}
@@ Line 30: / Line 32: @@
 [[Category: Yokota, H.]]
 [[Category: Zarembinski, T I.]]
-[[Category: ATP]]
-[[Category: MN]]
 [[Category: atp binding protein]]
 [[Category: berkeley structural genomics center]]
@@ Line 41: / Line 41: @@
 [[Category: structural genomic]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 23 12:46:13 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Mar 26 05:56:56 2008''