5c9h: Difference between revisions
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''' | ==Structural Basis of Template Boundary Definition in Tetrahymena Telomerase== | ||
<StructureSection load='5c9h' size='340' side='right' caption='[[5c9h]], [[Resolution|resolution]] 3.00Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[5c9h]] is a 6 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5C9H OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5C9H FirstGlance]. <br> | |||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> | |||
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/RNA-directed_DNA_polymerase RNA-directed DNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.49 2.7.7.49] </span></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5c9h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5c9h OCA], [http://pdbe.org/5c9h PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5c9h RCSB], [http://www.ebi.ac.uk/pdbsum/5c9h PDBsum]</span></td></tr> | |||
</table> | |||
== Function == | |||
[[http://www.uniprot.org/uniprot/TERT_TETTH TERT_TETTH]] Telomerase is a ribonucleoprotein enzyme essential for the replication of chromosome termini in most eukaryotes. It elongates telomeres. It is a reverse transcriptase that adds simple sequence repeats to chromosome ends by copying a template sequence within the RNA component of the enzyme (By similarity). | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Telomerase is required to maintain repetitive G-rich telomeric DNA sequences at chromosome ends. To do so, the telomerase reverse transcriptase (TERT) subunit reiteratively uses a small region of the integral telomerase RNA (TER) as a template. An essential feature of telomerase catalysis is the strict definition of the template boundary to determine the precise TER nucleotides to be reverse transcribed by TERT. We report the 3-A crystal structure of the Tetrahymena TERT RNA-binding domain (tTRBD) bound to the template boundary element (TBE) of TER. tTRBD is wedged into the base of the TBE RNA stem-loop, and each of the flanking RNA strands wraps around opposite sides of the protein domain. The structure illustrates how the tTRBD establishes the template boundary by positioning the TBE at the correct distance from the TERT active site to prohibit copying of nontemplate nucleotides. | |||
Structural basis of template-boundary definition in Tetrahymena telomerase.,Jansson LI, Akiyama BM, Ooms A, Lu C, Rubin SM, Stone MD Nat Struct Mol Biol. 2015 Oct 5. doi: 10.1038/nsmb.3101. PMID:26436828<ref>PMID:26436828</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 5c9h" style="background-color:#fffaf0;"></div> | |||
[[Category: | == References == | ||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: RNA-directed DNA polymerase]] | |||
[[Category: Akiyama, B M]] | |||
[[Category: Jansson, L I]] | |||
[[Category: Lu, C]] | |||
[[Category: Ooms, A]] | [[Category: Ooms, A]] | ||
[[Category: | [[Category: Rubin, S M]] | ||
[[Category: | [[Category: Stone, M D]] | ||
[[Category: | [[Category: Rna binding protein-rna complex]] | ||
[[Category: | [[Category: Rna-protein complex]] | ||
[[Category: | [[Category: Telomerase]] | ||
Revision as of 06:56, 16 October 2015
Structural Basis of Template Boundary Definition in Tetrahymena TelomeraseStructural Basis of Template Boundary Definition in Tetrahymena Telomerase
Structural highlights
Function[TERT_TETTH] Telomerase is a ribonucleoprotein enzyme essential for the replication of chromosome termini in most eukaryotes. It elongates telomeres. It is a reverse transcriptase that adds simple sequence repeats to chromosome ends by copying a template sequence within the RNA component of the enzyme (By similarity). Publication Abstract from PubMedTelomerase is required to maintain repetitive G-rich telomeric DNA sequences at chromosome ends. To do so, the telomerase reverse transcriptase (TERT) subunit reiteratively uses a small region of the integral telomerase RNA (TER) as a template. An essential feature of telomerase catalysis is the strict definition of the template boundary to determine the precise TER nucleotides to be reverse transcribed by TERT. We report the 3-A crystal structure of the Tetrahymena TERT RNA-binding domain (tTRBD) bound to the template boundary element (TBE) of TER. tTRBD is wedged into the base of the TBE RNA stem-loop, and each of the flanking RNA strands wraps around opposite sides of the protein domain. The structure illustrates how the tTRBD establishes the template boundary by positioning the TBE at the correct distance from the TERT active site to prohibit copying of nontemplate nucleotides. Structural basis of template-boundary definition in Tetrahymena telomerase.,Jansson LI, Akiyama BM, Ooms A, Lu C, Rubin SM, Stone MD Nat Struct Mol Biol. 2015 Oct 5. doi: 10.1038/nsmb.3101. PMID:26436828[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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