PLC beta 3 Gq: Difference between revisions
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== Structural highlights == | == Structural highlights == | ||
'''Overview of the PLC-β3 and | '''Overview of the PLC-β3 and Gαq interface:''' | ||
The Gαq subunit consists two domains, one is the GTPase domain and the other is the alpha helical domain. These domains include three regions called <scene name='70/701452/Fig2/6'>switch regions I-III</scene>.These regions allows the Gαq to be released from the receptor and activate distinct downstream effectors, which carry on the signal to downstream targets. A central effector of Gαq being the PLC-β3 enzyme. The switch regions I and II interact with several domains in PLC-β3. | The Gαq subunit consists two domains, one is the GTPase domain and the other is the alpha helical domain. These domains include three regions called <scene name='70/701452/Fig2/6'>switch regions I-III</scene>.These regions allows the Gαq to be released from the receptor and activate distinct downstream effectors, which carry on the signal to downstream targets. A central effector of Gαq being the PLC-β3 enzyme. The switch regions I and II interact with several domains in PLC-β3. | ||
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PLC- β3 engages Gαq throughout three regions. First, an extended loop between the third and fourth EF hands of PLC-β3 directly supports switch residues critical for GTP hydrolysis by Gαq. Second, the region of PLC-β3 that connects the catalytic TIM barrel and the C2 domain interacts with both switches 1 and 2 of Gαq. Third, a segment composed of a helix-turn-helix at the C terminus of the C2 domain mostly located within a shallow declivity on the surface of Gαq formed by switch 2 and α3. | PLC- β3 engages Gαq throughout three regions. First, an extended loop between the third and fourth EF hands of PLC-β3 directly supports switch residues critical for GTP hydrolysis by Gαq. Second, the region of PLC-β3 that connects the catalytic TIM barrel and the C2 domain interacts with both switches 1 and 2 of Gαq. Third, a segment composed of a helix-turn-helix at the C terminus of the C2 domain mostly located within a shallow declivity on the surface of Gαq formed by switch 2 and α3. | ||
'''Local motifs in | '''Local motifs in Gαq-PLC-β3 interactions:''' | ||
<scene name='70/701452/Fig6/5'>The 703-725 loop</scene> between the end of the TIM barrel and the beginning of the C2 domain comprises a second distinct segment of PLC-β3 that makes extensive contacts with active Gαq, including switches 1 and 2. This interface includes a series of interdigitated pairs of charged residues, specifically in PLC-β3-Gαq Asp709/Arg202, Lys710/Glu191, and Asp721/Lys41; these in turn are supported by additional charged residues Glu703 and Arg707 of PLC- β3. | <scene name='70/701452/Fig6/5'>The 703-725 loop</scene> between the end of the TIM barrel and the beginning of the C2 domain comprises a second distinct segment of PLC-β3 that makes extensive contacts with active Gαq, including switches 1 and 2. This interface includes a series of interdigitated pairs of charged residues, specifically in PLC-β3-Gαq Asp709/Arg202, Lys710/Glu191, and Asp721/Lys41; these in turn are supported by additional charged residues Glu703 and Arg707 of PLC- β3. | ||