PLC beta 3 Gq: Difference between revisions
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<scene name='70/701452/Fig7/1'>An extended loop (residues 254-268)</scene> between EF hands 3 and 4 of PLC-β3 interacts with the GTP-binding region of Gαq. Asn260 of the EF3/4 loop promotes GTP hydrolysis by interaction with the side chain of Gln209 of Gαq, which rearranges during GTP hydrolysis to stabilize the transition state mimicked by GDP•AlF4–•H20. Asn260 also interacts with Glu212 to stabilize switch 1 for GTP hydrolysis. | <scene name='70/701452/Fig7/1'>An extended loop (residues 254-268)</scene> between EF hands 3 and 4 of PLC-β3 interacts with the GTP-binding region of Gαq. Asn260 of the EF3/4 loop promotes GTP hydrolysis by interaction with the side chain of Gln209 of Gαq, which rearranges during GTP hydrolysis to stabilize the transition state mimicked by GDP•AlF4–•H20. Asn260 also interacts with Glu212 to stabilize switch 1 for GTP hydrolysis. | ||
Other effectors are known to engage <scene name='70/701452/Fig3/3'>different regions (pink)</scene | Other effectors are known to engage <scene name='70/701452/Fig3/3'>different regions (pink)</scene> in Gαq within the Gα subunits. There are a large family of regulator of G protein signaling (RGS) proteins that independently accelerates the GTP hydrolysis , these RGS proteins engage the GTPase domain throughout <scene name='70/701452/Fig4/3'>several residues (brown)</scene> on Gαq. PLC-β3 interacts with <scene name='70/701452/Fig5/4'>residues (blue)</scene> on Gαq that overlaps almost completely with portions of Gα subunits needed for engagement of RGS proteins and other effectors. <ref>PMID:20966218</ref> | ||
'''Critical residues in the interface:''' | '''Critical residues in the interface:''' | ||