PLC beta 3 Gq: Difference between revisions
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'''Local motifs in Gq-PLC-β3 interactions:''' | '''Local motifs in Gq-PLC-β3 interactions:''' | ||
<scene name='70/701452/Fig6/5'>The loop</scene> | <scene name='70/701452/Fig6/5'>The residues 703-725 loop</scene> between the end of the TIM barrel and the beginning of the C2 domain comprises a second distinct segment of PLC-β3 that makes extensive contacts with active Gαq, including switches 1 and 2. This interface includes a series of interdigitated pairs of charged residues, specifically in PLC-β3-Gαq Asp709/Arg202, Lys710/Glu191, and Asp721/Lys41; these in turn are supported by additional charged residues Glu703 and Arg707 of PLC- β3. | ||
<scene name='70/701452/Fig7/1'>An extended loop</scene> (residues 254-268) between EF hands 3 and 4 of PLC-β3 interacts with the GTP-binding region of Gαq. Asn260 of the EF3/4 loop promotes GTP hydrolysis by interaction with the side chain of Gln209 of Gαq, which rearranges during GTP hydrolysis to stabilize the transition state mimicked by GDP•AlF4–•H20. Asn260 also interacts with Glu212 to stabilize switch 1 for GTP hydrolysis. | <scene name='70/701452/Fig7/1'>An extended loop</scene> (residues 254-268) between EF hands 3 and 4 of PLC-β3 interacts with the GTP-binding region of Gαq. Asn260 of the EF3/4 loop promotes GTP hydrolysis by interaction with the side chain of Gln209 of Gαq, which rearranges during GTP hydrolysis to stabilize the transition state mimicked by GDP•AlF4–•H20. Asn260 also interacts with Glu212 to stabilize switch 1 for GTP hydrolysis. | ||