5c7u: Difference between revisions
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''' | ==5'-monophosphate wt Guanine Riboswitch bound to hypoxanthine.== | ||
<StructureSection load='5c7u' size='340' side='right' caption='[[5c7u]], [[Resolution|resolution]] 3.05Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[5c7u]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5C7U OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5C7U FirstGlance]. <br> | |||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HPA:HYPOXANTHINE'>HPA</scene>, <scene name='pdbligand=NCO:COBALT+HEXAMMINE(III)'>NCO</scene></td></tr> | |||
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5c7w|5c7w]]</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5c7u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5c7u OCA], [http://www.rcsb.org/pdb/explore.do?structureId=5c7u RCSB], [http://www.ebi.ac.uk/pdbsum/5c7u PDBsum]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
As one of its goals, synthetic biology seeks to increase the number of building blocks in nucleic acids. While efforts towards this goal are well advanced for DNA, they have hardly begun for RNA. Herein, we present a crystal structure for an RNA riboswitch where a stem C:G pair has been replaced by a pair between two components of an artificially expanded genetic-information system (AEGIS), Z and P, (6-amino-5-nitro-2(1H)-pyridone and 2-amino-imidazo[1,2-a]-1,3,5-triazin-4-(8H)-one). The structure shows that the Z:P pair does not greatly change the conformation of the RNA molecule nor the details of its interaction with a hypoxanthine ligand. This was confirmed in solution by in-line probing, which also measured a 3.7 nM affinity of the riboswitch for guanine. These data show that the Z:P pair mimics the natural Watson-Crick geometry in RNA in the first example of a crystal structure of an RNA molecule that contains an orthogonal added nucleobase pair. | |||
A Crystal Structure of a Functional RNA Molecule Containing an Artificial Nucleobase Pair.,Hernandez AR, Shao Y, Hoshika S, Yang Z, Shelke SA, Herrou J, Kim HJ, Kim MJ, Piccirilli JA, Benner SA Angew Chem Int Ed Engl. 2015 Aug 17;54(34):9853-6. doi: 10.1002/anie.201504731., Epub 2015 Jul 16. PMID:26223188<ref>PMID:26223188</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
== References == | |||
[[Category: | <references/> | ||
__TOC__ | |||
</StructureSection> | |||
[[Category: Benner, S A]] | |||
[[Category: Hernandez, A R]] | |||
[[Category: Herrou, J]] | |||
[[Category: Hoshika, S]] | |||
[[Category: Kim, H J]] | |||
[[Category: Kim, M J]] | |||
[[Category: Piccirilli, J A]] | |||
[[Category: Shao, Y]] | |||
[[Category: Shelke, S A]] | |||
[[Category: Yang, Z]] | [[Category: Yang, Z]] | ||
[[Category: | [[Category: Expanded rna]] | ||
[[Category: | [[Category: Riboswitch]] | ||
[[Category: | [[Category: Rna]] | ||
[[Category: | [[Category: Unnatural base pair]] | ||
Revision as of 17:37, 12 August 2015
5'-monophosphate wt Guanine Riboswitch bound to hypoxanthine.5'-monophosphate wt Guanine Riboswitch bound to hypoxanthine.
Structural highlights
Publication Abstract from PubMedAs one of its goals, synthetic biology seeks to increase the number of building blocks in nucleic acids. While efforts towards this goal are well advanced for DNA, they have hardly begun for RNA. Herein, we present a crystal structure for an RNA riboswitch where a stem C:G pair has been replaced by a pair between two components of an artificially expanded genetic-information system (AEGIS), Z and P, (6-amino-5-nitro-2(1H)-pyridone and 2-amino-imidazo[1,2-a]-1,3,5-triazin-4-(8H)-one). The structure shows that the Z:P pair does not greatly change the conformation of the RNA molecule nor the details of its interaction with a hypoxanthine ligand. This was confirmed in solution by in-line probing, which also measured a 3.7 nM affinity of the riboswitch for guanine. These data show that the Z:P pair mimics the natural Watson-Crick geometry in RNA in the first example of a crystal structure of an RNA molecule that contains an orthogonal added nucleobase pair. A Crystal Structure of a Functional RNA Molecule Containing an Artificial Nucleobase Pair.,Hernandez AR, Shao Y, Hoshika S, Yang Z, Shelke SA, Herrou J, Kim HJ, Kim MJ, Piccirilli JA, Benner SA Angew Chem Int Ed Engl. 2015 Aug 17;54(34):9853-6. doi: 10.1002/anie.201504731., Epub 2015 Jul 16. PMID:26223188[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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