4ynh: Difference between revisions
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==Structure of the C. elegans SAS-5 Implico dimerization domain== | ==Structure of the C. elegans SAS-5 Implico dimerization domain== | ||
<StructureSection load='4ynh' size='340' side='right' caption='[[4ynh]], [[Resolution|resolution]] 1.00Å' scene=''> | <StructureSection load='4ynh' size='340' side='right'caption='[[4ynh]], [[Resolution|resolution]] 1.00Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[4ynh]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4YNH OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4YNH FirstGlance]. <br> | <table><tr><td colspan='2'>[[4ynh]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Caeel Caeel]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4YNH OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4YNH FirstGlance]. <br> | ||
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ynh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ynh OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4ynh RCSB], [http://www.ebi.ac.uk/pdbsum/4ynh PDBsum]</span></td></tr> | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">sas-5, F35B12.5 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=6239 CAEEL])</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ynh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ynh OCA], [http://pdbe.org/4ynh PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4ynh RCSB], [http://www.ebi.ac.uk/pdbsum/4ynh PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4ynh ProSAT]</span></td></tr> | |||
</table> | </table> | ||
== Function == | == Function == | ||
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
</div> | </div> | ||
<div class="pdbe-citations 4ynh" style="background-color:#fffaf0;"></div> | |||
== References == | == References == | ||
<references/> | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Caeel]] | |||
[[Category: Large Structures]] | |||
[[Category: Hatzopoulos, G N]] | [[Category: Hatzopoulos, G N]] | ||
[[Category: Rogala, K B]] | [[Category: Rogala, K B]] | ||
Revision as of 09:34, 12 June 2019
Structure of the C. elegans SAS-5 Implico dimerization domainStructure of the C. elegans SAS-5 Implico dimerization domain
Structural highlights
Function[SAS5_CAEEL] Required for centrosome duplication. Essential for daughter-centriole formation. Requires both maternal and partenal expression, suggesting that it regulates centriole duplication during both spermatogenesis and early embryogenesis.[1] [2] Publication Abstract from PubMedCentrioles are microtubule-based organelles crucial for cell division, sensing and motility. In C. elegans, the onset of centriole formation requires notably the proteins SAS-5 and SAS-6, which have functional homologs across eukaryotic evolution. Whereas the molecular architecture of SAS-6 and its role in initiating centriole formation are well understood, the mechanisms by which SAS-5 and its relatives function is unclear. Here, we combine biophysical and structural analysis to uncover the architecture of SAS-5 and examine its functional implications in vivo. Our work reveals that two distinct self-associating domains are necessary to form higher-order oligomers of SAS-5: a trimeric coiled coil and a novel globular dimeric Implico domain. Disruption of either domain leads to centriole duplication failure in worm embryos, indicating that large SAS-5 assemblies are necessary for function in vivo. The protein SAS-5 forms large oligomeric assemblies critical for centriole formation.,Rogala KB, Dynes NJ, Hatzopoulos GN, Yan J, Pong SK, Robinson CV, Deane CM, Gonczy P, Vakonakis I Elife. 2015 May 29;4. doi: 10.7554/eLife.07410. PMID:26023830[3] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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