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| {{Sandbox_Reserved_Butler_CH462_Sp2015_#}}<!-- PLEASE ADD YOUR CONTENT BELOW HERE --> | | {{Sandbox_Reserved_Butler_CH462_Sp2015_#}}<!-- PLEASE ADD YOUR CONTENT BELOW HERE --> |
| == Background == | | ==Your Protein Name here== |
| | <StructureSection load='1stp' size='340' side='right' caption='Caption for this structure' scene=''> |
| | This is a default text for your page ''''''. Click above on '''edit this page''' to modify. Be careful with the < and > signs. |
| | You may include any references to papers as in: the use of JSmol in Proteopedia <ref>DOI 10.1002/ijch.201300024</ref> or to the article describing Jmol <ref>PMID:21638687</ref> to the rescue. |
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| The antigen 85 (ag85) complex in Mycobacterium tuberculosis is composed of three intracellular membrane proteins: ag85A, B, and C. The ag85 complex is a major component of the cell wall, with each protein catalyzing the transfer of important cell wall constituents into the membrane. <ref>PMID: 10655617</ref> Ag85C is of particular interest due to its transfer of mycolic acids, which are major components in determining cell wall integrity. By targeting this mycoloyltransferase activity, inhibition of ag85C offers potential for cell wall disruption and subsequent antibiotic targeting for normally drug-resistant mycotaberia tuberculosis. <ref>PMID: 10200974</ref>
| | == Biological Function == |
| ==Structure== | |
| <StructureSection load='1dqz' size='400' side='right' caption='Antigen 85C in Mycobacterium Tuberculosis' scene=''>
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| == General Structure == | | == Structural Overview == |
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| Antigen 85C was crystallized in its dimeric form.<ref>PMID: 25028518</ref> The <scene name='69/694220/Secondary_structures/2'>secondary structure</scene> shown in the monomeric form is composed of helices with one interwoven beta sheet. Due to the serine hydrolase activity of Ag85C, the enzyme contains a ‡/? hydrolase fold with a central ?-sheet bordered by ‡?helices, and this tertiary conformation is highly conserved across enzymes that function in this capacity. <ref>PMID:10655617</ref> The substrate binding pocket of Ag85C is composed of two separate but equally important components; there is carbohydrate binding pocket for the trehalose, and there is a fatty acid binding pocket for the mycolic acid. As a result, trahalose monomycolate can effectively bind to the Ag85C binding pocket.
| | == Mechanism of Action == |
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| == Enzymatic Activity == | | == Zinc Ligand(s) == |
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| Mutagenesis studies have confirmed the Ag85C functions through a Glu-His-Ser <scene name='69/694220/Catalytic_triad/2'>catalytic triad</scene>, similar to that of chymotrypsin. By modifying each of the catalytic residues separately testing the enzyme?s relative activity, it has been shown that mutation of any one of these residues dramatically reduces activity (Figure #). The S124 alcohol?s nucleophilicity is inductively strengthened through H260 and E224, which allows S124 to hydrolyze trehalose 6, 6?-dimycolate. The formation of the functional catalytic triad relies on upon Van der Waals interaction between C209 and the peptide bond between L232 and T231. This interaction results in a kinked conformation of the ‡9 helix, which promotes that activity of the catalytic triad. As a result, Ag85C, a mycolyl transferase, can facilitate the modification of trehalose monomycolates to trehalose dimycolates, which are then transported to the bacterial cell wall.
| | == Other Ligands == |
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| [[Image:General_mechanism.jpg|400 px|center|General reaction catalyzed by Antigen 85C]]
| | This is a sample scene created with SAT to <scene name="/12/3456/Sample/1">color</scene> by Group, and another to make <scene name="/12/3456/Sample/2">a transparent representation</scene> of the protein. You can make your own scenes on SAT starting from scratch or loading and editing one of these sample scenes. |
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| == Methods of Inhibition ==
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| [[Image:C209.jpeg|200 px|left|thumb|Cys209 stabilizing kinked formation of alpha-9 helix]]
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| Due to the importance of Ag85C enzymatic activity in maintaining the integrity of the mycobacteria tuberculosis cell wall though mycolic acid modifications, the Ag85C enzyme represents a potentially effective avenue for inhibiting cell growth. The conformational sensitivity of the active site residues, H260, E228, and S124, relies entirely upon Van der Waals interaction between C209 and L232-T 231 (Figure #). The C209 facilitated interaction causes the <scene name='69/694220/Alpha_9_helix/2'>‡9 helix</scene> to acquire a kinked conformation that promotes optimal interaction distances between catalytic residues. As a result, C209 has been a specific target residue for Ag85C inhibition.>
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| [[Image:Ebselen_inhibition.jpeg|200 px|right|thumb|Ebselen inhibition relaxing the alpha-9 helix]]
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| Ag85C can be inhibited by ebselen covalently bound to the sulfur of C209. Ebselen is a thiol-modifying agent that serves as an electrophile for the C209 that results in a sulfur-selenium bond. Co-crystallization of ebselen with Ag85C provides an explanation for the mechanism of ebselen-based inhibition. The addition of ebselen increases the distance between C209 and L232-T31, which effectively disrupts the interaction that holds the ‡9 helix in the active conformation. Furthermore, the bulk of ebselen creates steric hindrance with the ‡9 helix residues (Figure #). Relaxation of the ‡9 helix removes E228 and H260, which now interacts with S148, from the active site. The absence of these residues decreases the nucleophilicity of the S124 alcohol which decreases serine hydrolytic activity.
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| ===Inhibitors===
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| Additional thiol-modifying agents, p-chloromercuribenzoic acid and iodoacetamide, were crystalized with Ag85C. The structures show that each of these thiol-reactive inhibitors covalently bound to C209 and caused a relaxation of the ‡9 helix in a similar fashion to ebselen.
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| [[Image:Inhibitors_Ag85c.jpeg|400 px|center]]
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| </StructureSection> | | </StructureSection> |
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| == References == | | == References == |
| <references/> | | <references/> |