2v8n: Difference between revisions
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|ACTIVITY= | |ACTIVITY= | ||
|GENE= | |GENE= | ||
|DOMAIN= | |||
|RELATEDENTRY=[[1pv6|1PV6]], [[1pv7|1PV7]], [[2cfp|2CFP]], [[1m2u|1M2U]], [[2cfq|2CFQ]] | |||
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2v8n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2v8n OCA], [http://www.ebi.ac.uk/pdbsum/2v8n PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2v8n RCSB]</span> | |||
}} | }} | ||
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[[Category: transport protein]] | [[Category: transport protein]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 05:09:36 2008'' | ||
Revision as of 05:09, 31 March 2008
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| , resolution 3.60Å | |||||||
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| Related: | 1PV6, 1PV7, 2CFP, 1M2U, 2CFQ
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| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
WILD-TYPE STRUCTURE OF LACTOSE PERMEASE
OverviewOverview
Here we describe an x-ray structure of wild-type lactose permease (LacY) from Escherichia coli determined by manipulating phospholipid content during crystallization. The structure exhibits the same global fold as the previous x-ray structures of a mutant that binds sugar but cannot catalyze translocation across the membrane. LacY is organized into two six-helix bundles with twofold pseudosymmetry separated by a large interior hydrophilic cavity open only to the cytoplasmic side and containing the side chains important for sugar and H(+) binding. To initiate transport, binding of sugar and/or an H(+) electrochemical gradient increases the probability of opening on the periplasmic side. Because the inward-facing conformation represents the lowest free-energy state, the rate-limiting step for transport may be the conformational change leading to the outward-facing conformation.
About this StructureAbout this Structure
2V8N is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
ReferenceReference
Structural determination of wild-type lactose permease., Guan L, Mirza O, Verner G, Iwata S, Kaback HR, Proc Natl Acad Sci U S A. 2007 Sep 25;104(39):15294-8. Epub 2007 Sep 19. PMID:17881559
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