5ahv: Difference between revisions

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-'''Unreleased structure'''
+==Cryo-EM structure of helical ANTH and ENTH tubules on PI(4,5)P2-containing membranes==
+<StructureSection load='5ahv' size='340' side='right' caption='[[5ahv]], [[Resolution|resolution]] 13.60&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[5ahv]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5AHV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5AHV FirstGlance]. <br>
+</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ahv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ahv OCA], [http://www.rcsb.org/pdb/explore.do?structureId=5ahv RCSB], [http://www.ebi.ac.uk/pdbsum/5ahv PDBsum]</span></td></tr>
+</table>
+== Function ==
+[[http://www.uniprot.org/uniprot/ENT1_YEAST ENT1_YEAST]] Binds to membranes enriched in phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2) and phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2). Required for endocytosis and localization of actin. Negatively regulated via phosphorylation.<ref>PMID:10449404</ref> <ref>PMID:11694597</ref> <ref>PMID:12529323</ref>  [[http://www.uniprot.org/uniprot/SLA2_YEAST SLA2_YEAST]] Required for cellular morphogenesis and polarization of the cortical cytoskeleton. It might act in concert with proteins such as CDC42 and CDC43 to limit the region of cortical patch formation to the cortex of the bud. Required for the accumulation and/or maintenance of plasma membrane H(+)-ATPase on the cell surface.
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Clathrin-mediated endocytosis, the main trafficking route from the plasma membrane to the cytoplasm, is critical to many fundamental cellular processes. Clathrin, coupled to the membrane by adaptor proteins, is thought to play a major structural role in endocytosis by self-assembling into a cage-like lattice around the forming vesicle. Although clathrin adaptors are essential for endocytosis, little is known about their structural role in this process. Here we show that the membrane-binding domains of two conserved clathrin adaptors, Sla2 and Ent1, co-assemble in a PI(4,5)P2-dependent manner to form organized lattices on membranes. We determined the structure of the co-assembled lattice by electron cryo-microscopy and designed mutations that specifically impair the lattice formation in vitro. We show that these mutations block endocytosis in vivo. We suggest that clathrin adaptors not only link the polymerized clathrin to the membrane but also form an oligomeric structure, which is essential for membrane remodeling during endocytosis.
-The entry 5ahv is ON HOLD  until Paper Publication
+An Organized Co-assembly of Clathrin Adaptors Is Essential for Endocytosis.,Skruzny M, Desfosses A, Prinz S, Dodonova SO, Gieras A, Uetrecht C, Jakobi AJ, Abella M, Hagen WJ, Schulz J, Meijers R, Rybin V, Briggs JA, Sachse C, Kaksonen M Dev Cell. 2015 Apr 20;33(2):150-62. doi: 10.1016/j.devcel.2015.02.023. PMID:25898165<ref>PMID:25898165</ref>
-Authors: Skruzny, M., Desfosses, A., Prinz, S., Dodonova, S.O., Gieras, A., Uetrecht, C., Jakobi, A.J., Abella, M., Hagen, W.J.H., Schulz, J., Meijers, R., Rybin, V., Briggs, J.A.G., Sachse, C., Kaksonen, M.
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
-Description: Cryo-EM structure of helical ANTH and ENTH tubules on PI(4,5)P2-containing membranes
+== References ==
-[[Category: Unreleased Structures]]
+<references/>
+__TOC__
+</StructureSection>
+[[Category: Abella, M]]
+[[Category: Briggs, J A.G]]
 [[Category: Desfosses, A]]
-[[Category: Abella, M]]
+[[Category: Dodonova, S O]]
-[[Category: Hagen, W.J.H]]
 [[Category: Gieras, A]]
+[[Category: Hagen, W J.H]]
+[[Category: Jakobi, A J]]
 [[Category: Kaksonen, M]]
-[[Category: Uetrecht, C]]
+[[Category: Meijers, R]]
-[[Category: Briggs, J.A.G]]
+[[Category: Prinz, S]]
 [[Category: Rybin, V]]
-[[Category: Meijers, R]]
+[[Category: Sachse, C]]
 [[Category: Schulz, J]]
-[[Category: Jakobi, A.J]]
-[[Category: Dodonova, S.O]]
-[[Category: Sachse, C]]
-[[Category: Prinz, S]]
 [[Category: Skruzny, M]]
+[[Category: Uetrecht, C]]
+[[Category: Clathrin adaptor]]
+[[Category: Clathrin binding protein]]
+[[Category: Clathrin-binding protein]]
+[[Category: Endocytosis]]
+[[Category: Enth]]
+[[Category: Epsin]]
+[[Category: Hip1r]]