Sandbox Reserved 992: Difference between revisions
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== Function and Mechanism == | == Function and Mechanism == | ||
[[Image:Beta-lactam.jpg|200px|thumb|left|A β-lactam antibiotic (Penicillin)]]Clinically, β-lactam antibiotics, characterized by their central chemical structure, are utilized to combat bacterial infections by targeting penicillin-binding proteins (PBPs), also known as transpeptidases. PBPs are enzymes that are located in the cell membrane and function in cross-linking to form the peptidoglycan layer. PBPs have a deprotonated serine which executes nucleophilic attack on the carbonyl carbon. The PBP is then covalently attached to one unit of peptidoglycan. The amino group of an alanine on a second unit of peptidoglycan then performs a second nucleophilic attack on the carbonyl carbon, resulting in two covalently cross-linked peptidoglycan units and the regeneration of the catalytic PBP.<ref>"Peptidoglycan cell wall." The University of Warwick. n.d. Web. 25 Jan 15<ref | [[Image:Beta-lactam.jpg|200px|thumb|left|A β-lactam antibiotic (Penicillin)]]Clinically, β-lactam antibiotics, characterized by their central chemical structure, are utilized to combat bacterial infections by targeting penicillin-binding proteins (PBPs), also known as transpeptidases. PBPs are enzymes that are located in the cell membrane and function in cross-linking to form the peptidoglycan layer. PBPs have a deprotonated serine which executes nucleophilic attack on the carbonyl carbon. The PBP is then covalently attached to one unit of peptidoglycan. The amino group of an alanine on a second unit of peptidoglycan then performs a second nucleophilic attack on the carbonyl carbon, resulting in two covalently cross-linked peptidoglycan units and the regeneration of the catalytic PBP.<ref>"Peptidoglycan cell wall." The University of Warwick. n.d. Web. 25 Jan 15</ref> | ||
[[Image:Peptidoglycan_cross_linking.png|500px|thumb|right|Peptidoglycan with PDB Cross-linking Mechanism]] | [[Image:Peptidoglycan_cross_linking.png|500px|thumb|right|Peptidoglycan with PDB Cross-linking Mechanism]] | ||