Sandbox Reserved 993: Difference between revisions
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Step 2: Luciferase then catalyzes O<sub>2</sub>, producing light and oxyluciferin from Luciferyl AMP. <ref>Thorne, N., Shen, M., Lea, W. A., Simeonov, A., Lovell, S., Auld, D. S. and Inglese, J. (2012) "Firefly luciferase in chemical biology: A compendium of inhibitor, mechanistic evaluation of chemotypes, and suggested use as a reporter", Chem. Biol. 19(8): 1060-1072. doi:http://dx.doi.org/10.1016%2Fj.chembiol.2012.07.015</ref><ref name=White1980 /> | Step 2: Luciferase then catalyzes O<sub>2</sub>, producing light and oxyluciferin from Luciferyl AMP. <ref>Thorne, N., Shen, M., Lea, W. A., Simeonov, A., Lovell, S., Auld, D. S. and Inglese, J. (2012) "Firefly luciferase in chemical biology: A compendium of inhibitor, mechanistic evaluation of chemotypes, and suggested use as a reporter", Chem. Biol. 19(8): 1060-1072. doi:http://dx.doi.org/10.1016%2Fj.chembiol.2012.07.015</ref><ref name=White1980 /> | ||
[[Image: Luciferase_Mechanism_Without_Spelling_Errors.jpg] | [[Image:http://proteopedia.org/wiki/images/8/84/Luciferase_Mechanism_Without_Spelling_Errors.jpg]] | ||
The active site environment influences the wavelength of the light emitted. Single amino acid changes within the active site of Photinus pyralis can shift the luminescence from yellow-green to red.<ref name=Auld2008 /> Modifying the position of the Ser314-Leu319 loop near the active site can alter Biolumanescence color. When assayed under acidic conditions, all spectra underwent a red shift while basic conditions caused a blue shift. These experiments were done using E. Coli as the host organism indicating that the internal pH of the cell was close to the external pH. These findings suggest a possible use of bioluminescence in pH monitoring, biosensing and tissue and animal imaging. (Shapiro, 2005) | The active site environment influences the wavelength of the light emitted. Single amino acid changes within the active site of Photinus pyralis can shift the luminescence from yellow-green to red.<ref name=Auld2008 /> Modifying the position of the Ser314-Leu319 loop near the active site can alter Biolumanescence color. When assayed under acidic conditions, all spectra underwent a red shift while basic conditions caused a blue shift. These experiments were done using E. Coli as the host organism indicating that the internal pH of the cell was close to the external pH. These findings suggest a possible use of bioluminescence in pH monitoring, biosensing and tissue and animal imaging. (Shapiro, 2005) | ||