4lw0: Difference between revisions
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==Structure of the THF riboswitch bound to adenine== | ==Structure of the THF riboswitch bound to adenine== | ||
<StructureSection load='4lw0' size='340' side='right' caption='[[4lw0]], [[Resolution|resolution]] 1.89Å' scene=''> | <StructureSection load='4lw0' size='340' side='right' caption='[[4lw0]], [[Resolution|resolution]] 1.89Å' scene=''> | ||
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADE:ADENINE'>ADE</scene></td></tr> | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADE:ADENINE'>ADE</scene></td></tr> | ||
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3sd3|3sd3]], [[4lvv|4lvv]], [[4lvw|4lvw]], [[4lvy|4lvy]], [[4lvx|4lvx]], [[4lvz|4lvz]]</td></tr> | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3sd3|3sd3]], [[4lvv|4lvv]], [[4lvw|4lvw]], [[4lvy|4lvy]], [[4lvx|4lvx]], [[4lvz|4lvz]]</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4lw0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4lw0 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4lw0 RCSB], [http://www.ebi.ac.uk/pdbsum/4lw0 PDBsum]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4lw0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4lw0 OCA], [http://pdbe.org/4lw0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4lw0 RCSB], [http://www.ebi.ac.uk/pdbsum/4lw0 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4lw0 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
</div> | </div> | ||
<div class="pdbe-citations 4lw0" style="background-color:#fffaf0;"></div> | |||
==See Also== | |||
*[[Riboswitch|Riboswitch]] | |||
== References == | == References == | ||
<references/> | <references/> | ||
Revision as of 21:04, 11 August 2016
Structure of the THF riboswitch bound to adenineStructure of the THF riboswitch bound to adenine
Structural highlights
Publication Abstract from PubMedThe tetrahydrofolate (THF) riboswitch regulates folate transport and metabolism in a number of Firmicutes by cooperatively binding two molecules of THF. To further understand this riboswitch's specificity for THF, binding and regulatory activity of a series of THF analogs and antifolates were examined. Our data reveal that although binding is dominated by the RNA's interactions with the pterin moiety, the para-aminobenzoic acid (pABA) moiety plays a significant role in transcriptional regulation. Further, we find that adenine and several other analogs bind with high affinity by an alternative binding mechanism. Despite a similar affinity to THF, adenine is a poor regulator of transcriptional attenuation. These results demonstrate that binding alone does not determine a compound's effectiveness in regulating the activity of the riboswitch-a complication in current efforts to develop antimicrobials that target these RNAs. A Disconnect between High-Affinity Binding and Efficient Regulation by Antifolates and Purines in the Tetrahydrofolate Riboswitch.,Trausch JJ, Batey RT Chem Biol. 2014 Feb 20;21(2):205-16. doi: 10.1016/j.chembiol.2013.11.012. Epub, 2014 Jan 2. PMID:24388757[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)
OCA- Batey, R T
- Trausch, J J
- Adenine binding
- Aptamer
- Bacillus subtili
- Bacterial
- Bacterial protein
- Base sequence
- Binding site
- Calorimetry
- Folic acid
- Gene expression regulation
- Genetic
- Guanine
- Leucovorin
- Ligand
- Magnesium
- Molecular sequence data
- Mrna
- Ncrna
- Nucleic acid conformation
- Nucleotide
- Point mutation
- Protein binding
- Protein structure
- Pseudoknot
- Regulation
- Riboswitch
- Rna
- S-adenosylmethionine
- Secondary
- Streptococcus mutan
- Terminator region
- Tetrahydrofolate
- Thermodynamic
- Three-way junction
- Transcription