Tachyplesin: Difference between revisions
No edit summary |
No edit summary |
||
| Line 51: | Line 51: | ||
<b><u> Clinical Importance </u></b> | <b><u> Clinical Importance </u></b> | ||
Unlike mammalian cell membrane, bacterial cell membrane are negatively charged. [http://en.wikipedia.org/wiki/Escherichia_coli <i>Escherichia coli</i>] and [http://en.wikipedia.org/wiki/Staphylococcus_aureus <i>Listeria monocytogenes</i>] being most common pathogenic bacteria in animals and humans, studying the effect of TP-I on <i>E. coli</i> and <i>S. aureus</i> will be valuable in guiding clinical practice. The study in <i>E. coli</i> has shown membrane disruption upon treatment with TP-I. Also macromolecule leakage into the cytoplasm and the release of potassium ions was observed that ultimately | Unlike mammalian cell membrane, bacterial cell membrane are negatively charged. [http://en.wikipedia.org/wiki/Escherichia_coli <i>Escherichia coli</i>] and [http://en.wikipedia.org/wiki/Staphylococcus_aureus <i>Listeria monocytogenes</i>] being most common pathogenic bacteria in animals and humans, studying the effect of TP-I on <i>E. coli</i> and <i>S. aureus</i> will be valuable in guiding clinical practice. The study in <i>E. coli</i> has shown membrane disruption upon treatment with TP-I. Also macromolecule leakage into the cytoplasm and the release of potassium ions was observed that ultimately killed <i>E. coli</i>. | ||
In food production, requirements must be met for producing high quality food with minimal microbial contamination, and the determination of microbial viability based on different physiological and metabolic parameters is critical for acceptable sterilization. Therefore, the presence of injured, metabolically active bacteria is a very important aspect to consider in food production and for clinical applications. Sub lethally injured cells might be repaired under suitable conditions. If TP-I is applied as a clinical treatment at a lower concentration than the [http://en.wikipedia.org/wiki/Minimum_inhibitory_concentration MIC] over a long period of time, drug resistance could develop.<ref name=Hong>Hong, Jun, et al. "Mechanism of tachyplesin I injury to bacterial membrane and intracellular enzymes, determined by laser confocal scanning microscopy and flow cytometry." Microbiological research (2014).</ref> | In food production, requirements must be met for producing high quality food with minimal microbial contamination, and the determination of microbial viability based on different physiological and metabolic parameters is critical for acceptable sterilization. Therefore, the presence of injured, metabolically active bacteria is a very important aspect to consider in food production and for clinical applications. Sub lethally injured cells might be repaired under suitable conditions. If TP-I is applied as a clinical treatment at a lower concentration than the [http://en.wikipedia.org/wiki/Minimum_inhibitory_concentration MIC] over a long period of time, drug resistance could develop.<ref name=Hong>Hong, Jun, et al. "Mechanism of tachyplesin I injury to bacterial membrane and intracellular enzymes, determined by laser confocal scanning microscopy and flow cytometry." Microbiological research (2014).</ref> | ||