2vk2: Difference between revisions
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==CRYSTAL STRUCTURE OF A GALACTOFURANOSE BINDING PROTEIN== | ==CRYSTAL STRUCTURE OF A GALACTOFURANOSE BINDING PROTEIN== | ||
<StructureSection load='2vk2' size='340' side='right' caption='[[2vk2]], [[Resolution|resolution]] 1.20Å' scene=''> | <StructureSection load='2vk2' size='340' side='right' caption='[[2vk2]], [[Resolution|resolution]] 1.20Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[2vk2]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/ | <table><tr><td colspan='2'>[[2vk2]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Ecoli Ecoli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VK2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2VK2 FirstGlance]. <br> | ||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GZL:BETA-D-GALACTOFURANOSE'>GZL</scene></td></tr> | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GZL:BETA-D-GALACTOFURANOSE'>GZL</scene></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2vk2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2vk2 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2vk2 RCSB], [http://www.ebi.ac.uk/pdbsum/2vk2 PDBsum]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2vk2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2vk2 OCA], [http://pdbe.org/2vk2 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2vk2 RCSB], [http://www.ebi.ac.uk/pdbsum/2vk2 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2vk2 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Evolutionary Conservation == | == Evolutionary Conservation == | ||
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<text>to colour the structure by Evolutionary Conservation</text> | <text>to colour the structure by Evolutionary Conservation</text> | ||
</jmolCheckbox> | </jmolCheckbox> | ||
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/ | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2vk2 ConSurf]. | ||
<div style="clear:both"></div> | <div style="clear:both"></div> | ||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
</div> | </div> | ||
<div class="pdbe-citations 2vk2" style="background-color:#fffaf0;"></div> | |||
==See Also== | ==See Also== | ||
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__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: | [[Category: Ecoli]] | ||
[[Category: Horler, R S.P]] | [[Category: Horler, R S.P]] | ||
[[Category: Muller, A]] | [[Category: Muller, A]] | ||
Revision as of 16:49, 11 August 2016
CRYSTAL STRUCTURE OF A GALACTOFURANOSE BINDING PROTEINCRYSTAL STRUCTURE OF A GALACTOFURANOSE BINDING PROTEIN
Structural highlights
Evolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMedThe widespread utilization of sugars by microbes is reflected in the diversity and multiplicity of cellular transporters used to acquire these compounds from the environment. The model bacterium Escherichia coli has numerous transporters that allow it to take up hexoses and pentoses, which recognize the more abundant pyranose forms of these sugars. Here we report the biochemical and structural characterization of a transporter protein YtfQ from E. coli that forms part of an uncharacterized ABC transporter system. Remarkably the crystal structure of this protein, solved to 1.2 A using x-ray crystallography, revealed that YtfQ binds a single molecule of galactofuranose in its ligand binding pocket. Selective binding of galactofuranose over galactopyranose was also observed using NMR methods that determined the form of the sugar released from the protein. The pattern of expression of the ytfQRTyjfF operon encoding this transporter mirrors that of the high affinity galactopyranose transporter of E. coli, suggesting that this bacterium has evolved complementary transporters that enable it to use all the available galactose present during carbon limiting conditions. Furanose-specific sugar transport: characterization of a bacterial galactofuranose-binding protein.,Horler RS, Muller A, Williamson DC, Potts JR, Wilson KS, Thomas GH J Biol Chem. 2009 Nov 6;284(45):31156-63. Epub 2009 Sep 10. PMID:19744923[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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