2npx: Difference between revisions
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|PDB= 2npx |SIZE=350|CAPTION= <scene name='initialview01'>2npx</scene>, resolution 2.4Å | |PDB= 2npx |SIZE=350|CAPTION= <scene name='initialview01'>2npx</scene>, resolution 2.4Å | ||
|SITE= | |SITE= | ||
|LIGAND= <scene name='pdbligand=CYO:OXYGENS+BOUND+TO+CYS+SG'>CYO</scene>, <scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene> | |LIGAND= <scene name='pdbligand=CYO:OXYGENS+BOUND+TO+CYS+SG'>CYO</scene>, <scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=NAD:NICOTINAMIDE-ADENINE-DINUCLEOTIDE'>NAD</scene> | ||
|ACTIVITY= [http://en.wikipedia.org/wiki/NADH_peroxidase NADH peroxidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.11.1.1 1.11.1.1] | |ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/NADH_peroxidase NADH peroxidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.11.1.1 1.11.1.1] </span> | ||
|GENE= | |GENE= | ||
|DOMAIN= | |||
|RELATEDENTRY= | |||
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2npx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2npx OCA], [http://www.ebi.ac.uk/pdbsum/2npx PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2npx RCSB]</span> | |||
}} | }} | ||
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[[Category: Schulz, G E.]] | [[Category: Schulz, G E.]] | ||
[[Category: Stehle, T.]] | [[Category: Stehle, T.]] | ||
[[Category: oxidoreductase(h2o2(a))]] | [[Category: oxidoreductase(h2o2(a))]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 04:06:37 2008'' | ||
Revision as of 04:06, 31 March 2008
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| , resolution 2.4Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , , | ||||||
| Activity: | NADH peroxidase, with EC number 1.11.1.1 | ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
NADH BINDING SITE AND CATALYSIS OF NADH PEROXIDASE
OverviewOverview
The structure of the complex between cofactor NADH and the enzyme NADH peroxidase from Streptococcus faecalis 10C1 (Enterococcus faecalis) has been determined by crystal soaking, X-ray data collection, model building of NADH and refinement at 0.24-nm resolution based on the known enzyme structure [Stehle, T., Ahmed, S. A., Claiborne, A. & Schulz, G. E. (1991) J. Mol. Biol. 221, 1325-1344]. Apart from NADH, the catalytic center of the enzyme contains FAD and a cysteine that shuttles between thiolate and sulfenic acid states. Unfortunately, this cysteine was irreversibly oxidized to a cysteine sulfonic acid in the established enzyme structure. Based on the geometry of the catalytic center, we discuss the stabilization of the oxidation-sensitive sulfenic acid and propose a reaction mechanism.
About this StructureAbout this Structure
2NPX is a Single protein structure of sequence from Enterococcus faecalis. Full crystallographic information is available from OCA.
ReferenceReference
NADH binding site and catalysis of NADH peroxidase., Stehle T, Claiborne A, Schulz GE, Eur J Biochem. 1993 Jan 15;211(1-2):221-6. PMID:8425532
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