2itj: Difference between revisions
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|LIGAND= | |LIGAND= | ||
|ACTIVITY= | |ACTIVITY= | ||
|GENE= large T antigen ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id= Simian | |GENE= large T antigen ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10633 Simian virus 40]) | ||
|DOMAIN= | |||
|RELATEDENTRY=[[2ipr|2IPR]], [[2itl|2ITL]], [[2nl8|2NL8]] | |||
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2itj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2itj OCA], [http://www.ebi.ac.uk/pdbsum/2itj PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2itj RCSB]</span> | |||
}} | }} | ||
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[[Category: dna binding protein]] | [[Category: dna binding protein]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 03:48:25 2008'' | ||
Revision as of 03:48, 31 March 2008
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| , resolution 2.5Å | |||||||
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| Gene: | large T antigen (Simian virus 40) | ||||||
| Related: | 2IPR, 2ITL, 2NL8
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| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Origin binding domain of the SV40 large T antigen (residues 131-259). P212121 crystal form
OverviewOverview
The large T antigen (T-ag) protein binds to and activates DNA replication from the origin of DNA replication (ori) in simian virus 40 (SV40). Here, we determined the crystal structures of the T-ag origin-binding domain (OBD) in apo form, and bound to either a 17 bp palindrome (sites 1 and 3) or a 23 bp ori DNA palindrome comprising all four GAGGC binding sites for OBD. The T-ag OBDs were shown to interact with the DNA through a loop comprising Ser147-Thr155 (A1 loop), a combination of a DNA-binding helix and loop (His203-Asn210), and Asn227. The A1 loop traveled back-and-forth along the major groove and accounted for most of the sequence-determining contacts with the DNA. Unexpectedly, in both T-ag-DNA structures, the T-ag OBDs bound DNA independently and did not make direct protein-protein contacts. The T-ag OBD was also captured bound to a non-consensus site ATGGC even in the presence of its canonical site GAGGC. Our observations taken together with the known biochemical and structural features of the T-ag-origin interaction suggest a model for origin unwinding.
About this StructureAbout this Structure
2ITJ is a Single protein structure of sequence from Simian virus 40. Full crystallographic information is available from OCA.
ReferenceReference
Structure of the origin-binding domain of simian virus 40 large T antigen bound to DNA., Bochkareva E, Martynowski D, Seitova A, Bochkarev A, EMBO J. 2006 Dec 13;25(24):5961-9. Epub 2006 Nov 30. PMID:17139255
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