2hn2: Difference between revisions
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|PDB= 2hn2 |SIZE=350|CAPTION= <scene name='initialview01'>2hn2</scene>, resolution 3.700Å | |PDB= 2hn2 |SIZE=350|CAPTION= <scene name='initialview01'>2hn2</scene>, resolution 3.700Å | ||
|SITE= | |SITE= | ||
|LIGAND= <scene name='pdbligand=CA:CALCIUM ION'>CA</scene> | |LIGAND= <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene> | ||
|ACTIVITY= | |ACTIVITY= | ||
|GENE= CorA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2336 Thermotoga maritima]) | |GENE= CorA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2336 Thermotoga maritima]) | ||
|DOMAIN= | |||
|RELATEDENTRY=[[2bbj|2BBJ]], [[2bbh|2BBH]], [[2hn1|2HN1]], [[2iub|2IUB]] | |||
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2hn2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2hn2 OCA], [http://www.ebi.ac.uk/pdbsum/2hn2 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2hn2 RCSB]</span> | |||
}} | }} | ||
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[[Category: Pai, E F.]] | [[Category: Pai, E F.]] | ||
[[Category: Payandeh, J.]] | [[Category: Payandeh, J.]] | ||
[[Category: | [[Category: divalent cation]] | ||
[[Category: integral membrane protein | [[Category: integral membrane protein]] | ||
[[Category: metal transporter protein]] | |||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 03:32:34 2008'' | ||
Revision as of 03:32, 31 March 2008
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| , resolution 3.700Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | |||||||
| Gene: | CorA (Thermotoga maritima) | ||||||
| Related: | 2BBJ, 2BBH, 2HN1, 2IUB
| ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal structure of the CorA Mg2+ transporter homologue from T. maritima in complex with divalent cations
OverviewOverview
We describe the CorA Mg(2+) transporter homologue from Thermotoga maritima in complex with 12 divalent cations at 3.7 A resolution. One metal is found near the universally conserved GMN motif, apparently stabilized within the transmembrane region. This portion of the selectivity filter might discriminate between the size and preferred coordination geometry of hydrated substrates. CorA may further achieve specificity by requiring the sequential dehydration of substrates along the length of its approximately 55 A long pore. Ten metal sites identified within the cytoplasmic funnel domain are linked to long extensions of the pore helices and regulate the transport status of CorA. We have characterized this region as an intrinsic divalent cation sensor and provide evidence that it functions as a Mg(2+)-specific homeostatic molecular switch. A proteolytic protection assay, biophysical data, and comparison to a soluble domain structure from Archaeoglobus fulgidus have revealed the potential reaction coordinate for this diverse family of transport proteins.
About this StructureAbout this Structure
2HN2 is a Single protein structure of sequence from Thermotoga maritima. Full crystallographic information is available from OCA.
ReferenceReference
A structural basis for Mg2+ homeostasis and the CorA translocation cycle., Payandeh J, Pai EF, EMBO J. 2006 Aug 23;25(16):3762-73. Epub 2006 Aug 10. PMID:16902408
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