2hl3: Difference between revisions
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|ACTIVITY= | |ACTIVITY= | ||
|GENE= DCTN1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]) | |GENE= DCTN1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]) | ||
|DOMAIN= | |||
|RELATEDENTRY=[[2coy|2COY]], [[1txq|1TXQ]], [[2hkn|2HKN]], [[2hl3|2HL3]], [[2hl5|2HL5]] | |||
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2hl3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2hl3 OCA], [http://www.ebi.ac.uk/pdbsum/2hl3 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2hl3 RCSB]</span> | |||
}} | }} | ||
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==Disease== | ==Disease== | ||
Known | Known disease associated with this structure: Neuropathy, distal hereditary motor, type VIIB OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=601143 601143]], Amyotrophic lateral sclerosis, susceptibility to OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=601143 601143]] | ||
==About this Structure== | ==About this Structure== | ||
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[[Category: alpha-tubulin]] | [[Category: alpha-tubulin]] | ||
[[Category: clip-170]] | [[Category: clip-170]] | ||
[[Category: cytoskeleton associated protein | [[Category: dynactin,cytoskeleton associated protein]] | ||
[[Category: eb1]] | [[Category: eb1]] | ||
[[Category: eey/f-coo- sequence motif]] | [[Category: eey/f-coo- sequence motif]] | ||
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[[Category: p150glued]] | [[Category: p150glued]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 03:31:50 2008'' | ||
Revision as of 03:31, 31 March 2008
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| , resolution 2.03Å | |||||||
|---|---|---|---|---|---|---|---|
| Gene: | DCTN1 (Homo sapiens) | ||||||
| Related: | 2COY, 1TXQ, 2HKN, 2HL3, 2HL5
| ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal structure of the A49M mutant CAP-Gly domain of human Dynactin-1 (p150-Glued) in complex with human EB1 C-terminal hexapeptide
OverviewOverview
Dynamic microtubule plus-end tracking protein (+TIP) networks are implicated in all functions of microtubules, but the molecular determinants of their interactions are largely unknown. Here, we have explored key binding modes of +TIPs by analyzing the interactions between selected CAP-Gly, EB-like, and carboxy-terminal EEY/F-COO(-) sequence motifs. X-ray crystallography and biophysical binding studies demonstrate that the beta2-beta3 loop of CAP-Gly domains determines EB-like motif binding specificity. They further show how CAP-Gly domains serve as recognition domains for EEY/F-COO(-) motifs, which represent characteristic and functionally important sequence elements in EB, CLIP-170, and alpha-tubulin. Our findings provide a molecular basis for understanding the modular interaction modes between alpha-tubulin, CLIPs, EB proteins, and the dynactin-dynein motor complex and suggest that multiple low-affinity binding sites in different combinations control dynamic +TIP networks at microtubule ends. They further offer insights into the structural consequences of genetic CAP-Gly domain defects found in severe human disorders.
DiseaseDisease
Known disease associated with this structure: Neuropathy, distal hereditary motor, type VIIB OMIM:[601143], Amyotrophic lateral sclerosis, susceptibility to OMIM:[601143]
About this StructureAbout this Structure
2HL3 is a Protein complex structure of sequences from Homo sapiens. Full crystallographic information is available from OCA.
ReferenceReference
Key interaction modes of dynamic +TIP networks., Honnappa S, Okhrimenko O, Jaussi R, Jawhari H, Jelesarov I, Winkler FK, Steinmetz MO, Mol Cell. 2006 Sep 1;23(5):663-71. PMID:16949363
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