4led: Difference between revisions
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==The Crystal Structure of Pyocin L1 bound to D-rhamnose at 2.37 Angstroms== | ==The Crystal Structure of Pyocin L1 bound to D-rhamnose at 2.37 Angstroms== | ||
<StructureSection load='4led' size='340' side='right' caption='[[4led]], [[Resolution|resolution]] 2.37Å' scene=''> | <StructureSection load='4led' size='340' side='right' caption='[[4led]], [[Resolution|resolution]] 2.37Å' scene=''> | ||
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=XXR:6-DEOXY-ALPHA-D-MANNOPYRANOSE'>XXR</scene></td></tr> | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=XXR:6-DEOXY-ALPHA-D-MANNOPYRANOSE'>XXR</scene></td></tr> | ||
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4le7|4le7]], [[4lea|4lea]]</td></tr> | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4le7|4le7]], [[4lea|4lea]]</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4led FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4led OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4led RCSB], [http://www.ebi.ac.uk/pdbsum/4led PDBsum]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4led FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4led OCA], [http://pdbe.org/4led PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4led RCSB], [http://www.ebi.ac.uk/pdbsum/4led PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4led ProSAT]</span></td></tr> | ||
</table> | </table> | ||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
</div> | </div> | ||
<div class="pdbe-citations 4led" style="background-color:#fffaf0;"></div> | |||
== References == | == References == | ||
<references/> | <references/> | ||
Revision as of 18:52, 11 August 2016
The Crystal Structure of Pyocin L1 bound to D-rhamnose at 2.37 AngstromsThe Crystal Structure of Pyocin L1 bound to D-rhamnose at 2.37 Angstroms
Structural highlights
Publication Abstract from PubMedLectin-like bacteriocins consist of tandem monocot mannose-binding domains and display a genus-specific killing activity. Here we show that pyocin L1, a novel member of this family from Pseudomonas aeruginosa, targets susceptible strains of this species through recognition of the common polysaccharide antigen (CPA) of P. aeruginosa lipopolysaccharide that is predominantly a homopolymer of d-rhamnose. Structural and biophysical analyses show that recognition of CPA occurs through the C-terminal carbohydrate-binding domain of pyocin L1 and that this interaction is a prerequisite for bactericidal activity. Further to this, we show that the previously described lectin-like bacteriocin putidacin L1 shows a similar carbohydrate-binding specificity, indicating that oligosaccharides containing d-rhamnose and not d-mannose, as was previously thought, are the physiologically relevant ligands for this group of bacteriocins. The widespread inclusion of d-rhamnose in the lipopolysaccharide of members of the genus Pseudomonas explains the unusual genus-specific activity of the lectin-like bacteriocins. Lectin-Like Bacteriocins from Pseudomonas spp. Utilise D-Rhamnose Containing Lipopolysaccharide as a Cellular Receptor.,McCaughey LC, Grinter R, Josts I, Roszak AW, Waloen KI, Cogdell RJ, Milner J, Evans T, Kelly S, Tucker NP, Byron O, Smith B, Walker D PLoS Pathog. 2014 Feb 6;10(2):e1003898. doi: 10.1371/journal.ppat.1003898., eCollection 2014 Feb. PMID:24516380[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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