3tmr: Difference between revisions
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==IrisFP, planar chromophore== | ==IrisFP, planar chromophore== | ||
<StructureSection load='3tmr' size='340' side='right' caption='[[3tmr]], [[Resolution|resolution]] 2.00Å' scene=''> | <StructureSection load='3tmr' size='340' side='right' caption='[[3tmr]], [[Resolution|resolution]] 2.00Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[3tmr]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/ | <table><tr><td colspan='2'>[[3tmr]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Lobhe Lobhe]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TMR OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3TMR FirstGlance]. <br> | ||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO3:SULFITE+ION'>SO3</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO3:SULFITE+ION'>SO3</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | ||
<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CR8:2-[1-AMINO-2-(1H-IMIDAZOL-5-YL)ETHYL]-1-(CARBOXYMETHYL)-4-[(4-OXOCYCLOHEXA-2,5-DIEN-1-YLIDENE)METHYL]-1H-IMIDAZOL-5-OLATE'>CR8</scene></td></tr> | <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CR8:2-[1-AMINO-2-(1H-IMIDAZOL-5-YL)ETHYL]-1-(CARBOXYMETHYL)-4-[(4-OXOCYCLOHEXA-2,5-DIEN-1-YLIDENE)METHYL]-1H-IMIDAZOL-5-OLATE'>CR8</scene></td></tr> | ||
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2vvh|2vvh]], [[2vvi|2vvi]], [[2vvj|2vvj]], [[1zux|1zux]], [[2btj|2btj]], [[3tmt|3tmt]]</td></tr> | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2vvh|2vvh]], [[2vvi|2vvi]], [[2vvj|2vvj]], [[1zux|1zux]], [[2btj|2btj]], [[3tmt|3tmt]]</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3tmr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tmr OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3tmr RCSB], [http://www.ebi.ac.uk/pdbsum/3tmr PDBsum]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3tmr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tmr OCA], [http://pdbe.org/3tmr PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3tmr RCSB], [http://www.ebi.ac.uk/pdbsum/3tmr PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3tmr ProSAT]</span></td></tr> | ||
</table> | </table> | ||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
</div> | </div> | ||
<div class="pdbe-citations 3tmr" style="background-color:#fffaf0;"></div> | |||
== References == | == References == | ||
<references/> | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: | [[Category: Lobhe]] | ||
[[Category: Adam, V]] | [[Category: Adam, V]] | ||
[[Category: Bourgeois, D]] | [[Category: Bourgeois, D]] | ||
Revision as of 20:41, 11 August 2016
IrisFP, planar chromophoreIrisFP, planar chromophore
Structural highlights
Publication Abstract from PubMedFluorescent proteins (FPs) of the green fluorescent protein family blink and bleach like all fluorophores. However, contrary to organic dyes, the mechanisms by which transient losses of fluorescence occur in FPs have received little attention. Here, we focus on the photoactivatable IrisFP, for which a transient non-fluorescent chromophoric state with distorted geometry was recently reported (Adam, V.; et al. J. Am. Chem. Soc.2009, 131, 18063). We investigated the chemical nature of this blinked state by employing quantum chemical/molecular mechanical calculations. Our findings suggest two previously unidentified dark states that display similar distorted chromophores with a transiently ruptured pi-electron system. Both are protonated at atom C(alpha) of the chromophore methylene bridge. Transient protonation may occur via proton transfer from the nearby Arg66 either in the triplet state T(1) after intersystem crossing or in an anionic radical (doublet) ground state. As Arg66 is conserved in green-to-red photoconvertible FPs, these dark states are predicted to be common to all these proteins. We also suggest that C(alpha) protonated dark states may accelerate photobleaching by favoring decarboxylation of the fully conserved Glu212. The Nature of Transient Dark States in a Photoactivatable Fluorescent Protein.,Roy A, Field MJ, Adam V, Bourgeois D J Am Chem Soc. 2011 Oct 31. PMID:22039963[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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