2gcv: Difference between revisions
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|PDB= 2gcv |SIZE=350|CAPTION= <scene name='initialview01'>2gcv</scene>, resolution 2.10Å | |PDB= 2gcv |SIZE=350|CAPTION= <scene name='initialview01'>2gcv</scene>, resolution 2.10Å | ||
|SITE= | |SITE= | ||
|LIGAND= <scene name='pdbligand= | |LIGAND= <scene name='pdbligand=A:ADENOSINE-5'-MONOPHOSPHATE'>A</scene>, <scene name='pdbligand=A23:ADENOSINE-5'-PHOSPHATE-2',3'-CYCLIC+PHOSPHATE'>A23</scene>, <scene name='pdbligand=C:CYTIDINE-5'-MONOPHOSPHATE'>C</scene>, <scene name='pdbligand=G:GUANOSINE-5'-MONOPHOSPHATE'>G</scene>, <scene name='pdbligand=MES:2-(N-MORPHOLINO)-ETHANESULFONIC+ACID'>MES</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=U:URIDINE-5'-MONOPHOSPHATE'>U</scene> | ||
|ACTIVITY= | |ACTIVITY= | ||
|GENE= | |GENE= | ||
|DOMAIN= | |||
|RELATEDENTRY=[[2gcs|2GCS]], [[2h0s|2H0S]], [[2h0w|2H0W]], [[2h0x|2H0X]], [[2h0z|2H0Z]] | |||
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2gcv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2gcv OCA], [http://www.ebi.ac.uk/pdbsum/2gcv PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2gcv RCSB]</span> | |||
}} | }} | ||
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[[Category: Amare, A R.Ferre-D.]] | [[Category: Amare, A R.Ferre-D.]] | ||
[[Category: Klein, D J.]] | [[Category: Klein, D J.]] | ||
[[Category: | [[Category: helix]] | ||
[[Category: | [[Category: pseudoknot]] | ||
[[Category: rna | [[Category: ribozyme]] | ||
[[Category: rna]] | |||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 03:14:55 2008'' | ||
Revision as of 03:14, 31 March 2008
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| , resolution 2.10Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , , , , , , | ||||||
| Related: | 2GCS, 2H0S, 2H0W, 2H0X, 2H0Z
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| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Post-cleavage state of the Thermoanaerobacter tengcongensis glmS ribozyme
OverviewOverview
The glmS ribozyme is the only natural catalytic RNA known to require a small-molecule activator for catalysis. This catalytic RNA functions as a riboswitch, with activator-dependent RNA cleavage regulating glmS messenger RNA expression. We report crystal structures of the glmS ribozyme in precleavage states that are unliganded or bound to the competitive inhibitor glucose-6-phosphate and in the postcleavage state. All structures superimpose closely, revealing a remarkably rigid RNA that contains a preformed active and coenzyme-binding site. Unlike other riboswitches, the glmS ribozyme binds its activator in an open, solvent-accessible pocket. Our structures suggest that the amine group of the glmS ribozyme-bound coenzyme performs general acid-base and electrostatic catalysis.
About this StructureAbout this Structure
2GCV is a Single protein structure of sequence from [1]. Full crystallographic information is available from OCA.
ReferenceReference
Structural basis of glmS ribozyme activation by glucosamine-6-phosphate., Klein DJ, Ferre-D'Amare AR, Science. 2006 Sep 22;313(5794):1752-6. PMID:16990543
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